ArrayExpress Uploader0human21pegIFNalpha-2aB2 (144h after pegIFNalpha-2a injection)20pegIFNalpha-2aB2 (144h after pegIFNalpha-2a injection)19pegIFNalpha-2aB2 (144h after pegIFNalpha-2a injection)18pegIFNalpha-2bB2 (144h after pegIFNalpha-2b injection)17pegIFNalpha-2bB2 (144h after pegIFNalpha-2b injection)16pegIFNalpha-2bB2 (144h after pegIFNalpha-2b injection)15pegIFNalpha-2bB2 (96h after pegIFNalpha-2b injection)14pegIFNalpha-2bB2 (96h after pegIFNalpha-2b injection)13pegIFNalpha-2bB2 (96h after pegIFNalpha-2b injection)12pegIFNalpha-2bB2 (48h after pegIFNalpha-2b injection)11pegIFNalpha-2bB2 (48h after pegIFNalpha-2b injection)10pegIFNalpha-2bB2 (48h after pegIFNalpha-2b injection)9pegIFNalpha-2bB2 (16h after pegIFNalpha-2b injection)8pegIFNalpha-2bB2 (16h after pegIFNalpha-2b injection)7pegIFNalpha-2bB2 (16h after pegIFNalpha-2b injection)21controlB1 (before treatment)20controlB1 (before treatment)19controlB1 (before treatment)18controlB1 (before treatment)17controlB1 (before treatment)16controlB1 (before treatment)15controlB1 (before treatment)14controlB1 (before treatment)13controlB1 (before treatment)12controlB1 (before treatment)11controlB1 (before treatment)10controlB1 (before treatment)9controlB1 (before treatment)8controlB1 (before treatment)7controlB1 (before treatment)2129arrayexpress_sid4This dataset is part of the paper: Pegylated interferon-α regulates hepatic gene expression by transient activation of the Jak-STAT...E-GEOD-48445/profile/8773/arrayexpressuploader330chronic hepatitis chepatitisliverserumsuperiorviral hepatitisDec.12, 2014Falsegene-expression-profiling-of-liver-biopsies-from-2E-GEOD-48445_A-AFFY-44Gene expression profiling of liver biopsies from 21 chronic hepatitis C patients undergoing antiviral therapyJul.11, 2014This dataset is part of the paper: Pegylated interferon-α regulates hepatic gene expression by transient activation of the Jak-STAT pathway; Dill MT et al; Journal of Clinical Investigation; in review Pegylated interferon-α (pegIFN-α) has replaced un-modified recombinant IFN-α for the treatment of chronic viral hepatitis because of its superior anti-viral efficacy that is generally attributed to improved pharmacokinetic properties. However, the pharmacodynamic effects of pegIFN-α in the liver have not been studied. We analyzed pegIFN-α induced signaling and gene regulation in paired liver biopsies obtained before treatment and during the first week after injection of pegIFN-α in 18 patients. Despite sustained high serum concentrations of pegIFN-α over the entire one-week dosing interval, IFN-α signaling through the Jak-STAT pathway occurs only during the first day. PegIFN-α induces hundreds of genes that can be classified into 4 clusters based on different temporal expression profiles. In all clusters, gene transcription is mainly driven by IFN stimulated gene factor 3 (ISGF3). IFN induced secondary transcription factors do not cause additional waves of gene expression. We could not confirm a role of un-phosphorylated STAT1 in prolonging IFN-α induced gene transcription. Collectively, our results reveal that the major effects of pegIFN-α in the liver are caused by an early and transient activation of ISGF3. Prolonging the serum half-life of IFN-α does not necessarily improve its pharmacodynamic properties. Paired liver biopsy samples were collected before and during the first week of pegylated interferon alpha treatment of 21 chronic hepatitis C patients. Total: 21 patients and 42 samples.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-48445http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-48445/samples/