{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM1160722": {"IMMUNOPHENOTYPE": "CD3+TCRValpha+ iNKT-cell"}}, {"GSM116072": {"IMMUNOPHENOTYPE": "KIR+CD3+CD56+ T-cell"}}, {"GSM1160720": {"IMMUNOPHENOTYPE": "KIR-CD3+CD56+ T-cell"}}, {"GSM1160719": {"IMMUNOPHENOTYPE": "CD3+CD56+ T-cell"}}, {"GSM1160718": {"IMMUNOPHENOTYPE": "CD3-CD56+ NK cell"}}, {"GSM1160717": {"IMMUNOPHENOTYPE": "CD3+CD56- T-cell"}}, {"GSM1160722": {"IMMUNOPHENOTYPE": "CD3+TCRValpha+ iNKT-cell"}}, {"GSM116072": {"IMMUNOPHENOTYPE": "KIR+CD3+CD56+ T-cell"}}, {"GSM1160720": {"IMMUNOPHENOTYPE": "KIR-CD3+CD56+ T-cell"}}, {"GSM1160719": {"IMMUNOPHENOTYPE": "CD3+CD56+ T-cell"}}, {"GSM1160718": {"IMMUNOPHENOTYPE": "CD3-CD56+ NK cell"}}, {"GSM1160717": {"IMMUNOPHENOTYPE": "CD3+CD56- T-cell"}}, {"GSM1160722": {"IMMUNOPHENOTYPE": "CD3+TCRValpha+ iNKT-cell"}}, {"GSM116072": {"IMMUNOPHENOTYPE": "KIR+CD3+CD56+ T-cell"}}, {"GSM1160720": {"IMMUNOPHENOTYPE": "KIR-CD3+CD56+ T-cell"}}, {"GSM1160719": {"IMMUNOPHENOTYPE": "CD3+CD56+ T-cell"}}, {"GSM1160718": {"IMMUNOPHENOTYPE": "CD3-CD56+ NK cell"}}, {"GSM1160717": {"IMMUNOPHENOTYPE": "CD3+CD56- T-cell"}}, {"GSM1160722": {"IMMUNOPHENOTYPE": "CD3+TCRValpha+ iNKT-cell"}}, {"GSM116072": {"IMMUNOPHENOTYPE": "KIR+CD3+CD56+ T-cell"}}, {"GSM1160720": {"IMMUNOPHENOTYPE": "KIR-CD3+CD56+ T-cell"}}, {"GSM1160719": {"IMMUNOPHENOTYPE": "CD3+CD56+ T-cell"}}, {"GSM1160718": {"IMMUNOPHENOTYPE": "CD3-CD56+ NK cell"}}, {"GSM1160717": {"IMMUNOPHENOTYPE": "CD3+CD56- T-cell"}}, {"GSM1160722": {"IMMUNOPHENOTYPE": "CD3+TCRValpha+ iNKT-cell"}}, {"GSM116072": {"IMMUNOPHENOTYPE": "KIR+CD3+CD56+ T-cell"}}, {"GSM1160720": {"IMMUNOPHENOTYPE": "KIR-CD3+CD56+ T-cell"}}, {"GSM1160719": {"IMMUNOPHENOTYPE": "CD3+CD56+ T-cell"}}, {"GSM1160718": {"IMMUNOPHENOTYPE": "CD3-CD56+ NK cell"}}, {"GSM1160717": {"IMMUNOPHENOTYPE": "CD3+CD56- T-cell"}}], "id": 2371, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "A small subset of T cells also expresses kiler-cell immunoglobulin-like receptors (KIRs). We find that KIR+ T cells primarily reside in...", "geo_gse_id": "E-GEOD-47855", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 30, "tags": ["cell", "genome", "immunoglobulin", "lymphocyte", "peripheral"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-47855_A-AFFY-44", "slug": "gene-expression-analysis-for-cd56-t-nk-cd56-t-cell", "geo_gds_id": "", "name": "Gene expression analysis for CD56- T, NK, CD56+ T cells, and iNKT cells", "created": "Jul.12, 2014", "summary": "A small subset of T cells also expresses kiler-cell immunoglobulin-like receptors (KIRs). We find that KIR+ T cells primarily reside in the CD56+ T population. However, little is known on how these cells are different from the conventional CD56- T, NK, and iNKT cells. We used microarray profiling to compare and determine the distinctive differences of CD56+ T cell and its KIR subsets when compared to the conventional CD56- T, NK and iNKT cells. Lymphocyte subsets were sorted from human peripheral blood mononuclear cells with FACSAriaII (BD Biosciences, San Jose, CA) using anti-CD3, anti-CD56, anti-CD14, anti-KIR2DL1, anti-KIR2DL2/3, anti-KIR3DL1 and anti-TCRValpha24 antibodies. The purity of CD3+CD56- T cells, CD3-CD56+ NK cells, CD3+CD56+ T cells, KIR-CD3+CD56+ T cells, and KIR+CD3+CD56+ T cells were more than 98% in all experiments. The purities of iNKT cells for TCRValpha24 and CD1d-tetramer were >95% and >90%, respectively. RNA pre-amplification, labeling and hybridization on Human Genome U133Plus 2.0 GeneChip array were performed in the St. Jude Hartwell Center for Bioinformatics & Biotechnology microarray core facility according to the manufacturer\u2019s instructions (Affymetrix, Santa Clara, CA).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-47855", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-47855/samples/"}