{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1159579": {"GENOTYPE": "wild type"}}, {"GSM1159579": {"GENOTYPE": "wild type"}}, {"GSM1159579": {"GENOTYPE": "wild type"}}, {"GSM1159579": {"GENOTYPE": "wild type"}}, {"GSM1159583": {"GENOTYPE": "Cebpg-/-"}}, {"GSM1159583": {"GENOTYPE": "Cebpg-/-"}}, {"GSM1159583": {"GENOTYPE": "Cebpg-/-"}}, {"GSM1159583": {"GENOTYPE": "Cebpg-/-"}}], "id": 7336, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "C/EBP\u03b2 is an important regulator of oncogene-induced senescence (OIS). Here we show that C/EBP\u03b3, a heterodimeric partner of C/EBP\u03b2 whose...", "geo_gse_id": "E-GEOD-47777", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 8, "tags": ["basal", "bone", "bone marrow", "lung"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "whole-genome-expression-data-comparison-between-wt", "geo_id_plat": "E-GEOD-47777_A-AFFY-45", "name": "Whole genome expression data comparison between WT and Cebpg-/- MEFs.", "created": "Nov.12, 2014", "summary": "C/EBP\u03b2 is an important regulator of oncogene-induced senescence (OIS). Here we show that C/EBP\u03b3, a heterodimeric partner of C/EBP\u03b2 whose biological functions are not well understood, inhibits cellular senescence. Cebpg-/- MEFs proliferated poorly, entered senescence prematurely, and expressed a pro-inflammatory gene signature, including elevated levels of senescence-associated secretory phenotype (SASP) genes whose induction by oncogenic stress requires C/EBP\u03b2. The senescence-suppressing activity of C/EBP\u03b3 required its ability to heterodimerize with C/EBP\u03b2. Covalently linked C/EBP\u03b2 homodimers (\u03b2~\u03b2) inhibited the proliferation and tumorigenicity of RasV12-transformed NIH3T3 cells, activated SASP gene expression, and recruited the CBP co-activator in a Ras-dependent manner, whereas \u03b3~\u03b2 heterodimers lacked these capabilities and efficiently rescued proliferation of Cebpg-/- MEFs. C/EBP\u03b2 depletion partially restored growth of C/EBP\u03b3-deficient cells, indicating that the increased levels of C/EBP\u03b2 homodimers in Cebpg-/- MEFs inhibit proliferation. The proliferative functions of C/EBP\u03b3 are not restricted to fibroblasts, as hematopoietic progenitors from Cebpg-/- bone marrow also displayed impaired growth. Furthermore, high CEBPG expression correlated with poorer clinical prognoses in several human cancers, and C/EBP\u03b3 depletion decreased proliferation and induced senescence in lung tumor cells. Our findings demonstrate that C/EBP\u03b3 neutralizes the cytostatic activity of C/EBP\u03b2 through heterodimerization, which prevents senescence and suppresses basal transcription of SASP genes. Mouse embryonic fibroblasts were isolated at E13.5 and propagated.  At passage 3 cells were plated with equal density and collected 48 hours later.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-47777", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-47777/samples/"}