ArrayExpress Uploaderarrayexpress_sidhumantransduced with p63+KLF4 expressing retrovirus and treated with high calciumtransduced with p63+KLF4 expressing retrovirus and treated with high calciumtransduced with p63+KLF4 expressing retrovirus and treated with low calciumtransduced with p63+KLF4 expressing retrovirus and treated with low calciumtransduced with p63+KLF4 expressing retrovirustransduced with p63+KLF4 expressing retrovirustransduced with LacZ expressing retrovirustransduced with LacZ expressing retrovirus223704To determine if fibroblasts could be reprogrammed to a keratinocyte phenotype p63+KLF4 or LacZ expressing retroviruses were transduced...E-GEOD-47746/profile/8773/arrayexpressuploader18Dec.12, 2014Falsegene-expression-data-of-fibroblasts-transduced-witE-GEOD-47746_A-AFFY-44Gene expression data of fibroblasts transduced with LacZ or p63+KLF4.Jul.11, 2014To determine if fibroblasts could be reprogrammed to a keratinocyte phenotype p63+KLF4 or LacZ expressing retroviruses were transduced into primary human neonatal fibroblasts. Global gene expression profiling using U133 plus 2.0 arrays were used to deteremine the extent of reprogramming to a keratinocyte phenoypte upon transduction with p63+KLF4. Fibroblasts transduced with p63+KLF4 were also treated +/- high calcium to determine if treatment with calcium could induce differentiation of these cells. Microarray analysis was also performed on cells treated +/- calcium. For gene expression profiling, cultured human fibroblasts were infected with LacZ or p63+KLF4 expressing retroviruses. p63+KLF4 cells were also treated +/- calcium. Microarray analysis using Affymetrix HG-U133 2.0 plus arrays was performed on duplicate samples.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-47746http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-47746/samples/