{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 7326, "factors": [{"GSM1154514": {"SORTED CELL FRACTION": "CD45.2+"}}, {"GSM1154515": {"SORTED CELL FRACTION": "CD45.1+"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "By investigating the germinal center (GC) formation in STAT6ko/WT bone marrow-mixed chimera we found that GC formation in type 2 immune...", "pubmed_id": 24777733, "geo_gse_id": "E-GEOD-47679", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 2, "tags": ["bone", "bone marrow", "cell", "lymph"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "b-cell-intrinsic-stat6-controls-the-germinal-cente", "geo_id_plat": "E-GEOD-47679_A-AFFY-45", "name": "B cell-intrinsic STAT6 controls the germinal center response in type 2 immunity", "created": "Nov.12, 2014", "summary": "By investigating the germinal center (GC) formation in STAT6ko/WT bone marrow-mixed chimera we found that GC formation in type 2 immune responses is dependent on B cell intrinsic expression of IL-4/IL-13-induced genes. We therefore used microarrays to find Stat6 dependent genes that are important for germinal center formation and/or organization after infection with the nematode Nippostrongylus brasiliensis (N. brasiliensis). Bone marrow of STAT6ko (CD45.2+) and WT (CD45.1+) were mixed and injected in lethaly irradiated WT (CD45.1+) mice. After 8 weeks, 5 Bone marrow-mixed chimera were infected with N. brasiliensis and draining lymph nodes were collected at day 14 after the infection and pooled. RNA was isolated from sort-purified CD45.1+ or CD45.2+ GC B cells (B220+CD38loGL-7hi).", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-47679", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-47679/samples/"}