ArrayExpress Uploader07326CD45.2+CD45.1+arrayexpress_sid6By investigating the germinal center (GC) formation in STAT6ko/WT bone marrow-mixed chimera we found that GC formation in type 2 immune...24777733E-GEOD-47679/profile/8773/arrayexpressuploader12bonebone marrowcelllymphDec.12, 2014Falseb-cell-intrinsic-stat6-controls-the-germinal-centeE-GEOD-47679_A-AFFY-45B cell-intrinsic STAT6 controls the germinal center response in type 2 immunityNov.12, 2014By investigating the germinal center (GC) formation in STAT6ko/WT bone marrow-mixed chimera we found that GC formation in type 2 immune responses is dependent on B cell intrinsic expression of IL-4/IL-13-induced genes. We therefore used microarrays to find Stat6 dependent genes that are important for germinal center formation and/or organization after infection with the nematode Nippostrongylus brasiliensis (N. brasiliensis). Bone marrow of STAT6ko (CD45.2+) and WT (CD45.1+) were mixed and injected in lethaly irradiated WT (CD45.1+) mice. After 8 weeks, 5 Bone marrow-mixed chimera were infected with N. brasiliensis and draining lymph nodes were collected at day 14 after the infection and pooled. RNA was isolated from sort-purified CD45.1+ or CD45.2+ GC B cells (B220+CD38loGL-7hi).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-47679mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-47679/samples/