{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1146537": {"CELL TYPE": "FACS-sorted CCK-EGFP intestinal cells"}}, {"GSM1146538": {"CELL TYPE": "non-EGFP intestinal cells (GFP negative)"}}, {"GSM1146537": {"CELL TYPE": "FACS-sorted CCK-EGFP intestinal cells"}}, {"GSM1146538": {"CELL TYPE": "non-EGFP intestinal cells (GFP negative)"}}, {"GSM1146538": {"CELL TYPE": "non-EGFP intestinal cells (GFP negative)"}}, {"GSM1146537": {"CELL TYPE": "FACS-sorted CCK-EGFP intestinal cells"}}], "id": 7309, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Cholecystokinin (CCK) is a satiety hormone produced by discrete enteroendocrine cells scattered among absorptive cells of the small...", "geo_gse_id": "E-GEOD-47196", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["cholecystokinin", "fatty acid", "hormone", "immunoglobulin", "intestine", "lipoprotein", "small intestine"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "immunoglobulin-like-domain-receptor-1-mediates-fat", "geo_id_plat": "E-GEOD-47196_A-AFFY-45", "name": "Immunoglobulin-like domain receptor 1 mediates fat-stimulated cholecystokinin secretion.", "created": "Nov.12, 2014", "summary": "Cholecystokinin (CCK) is a satiety hormone produced by discrete enteroendocrine cells scattered among absorptive cells of the small intestine. CCK is released into blood following a meal; however, the mechanisms inducing hormone secretion are largely unknown. Ingested fat is the major stimulant of CCK secretion. We recently identified a novel member of the lipoprotein remnant receptor family known as immunoglobulin-like domain containing receptor 1 (ILDR1) in intestinal CCK cells and postulated that this receptor conveyed the signal for fat-stimulated CCK secretion. In the intestine, ILDR1 is expressed exclusively in CCK cells. Orogastric administration of fatty acids elevated blood levels of CCK in wild type but not ILDR1-deficient mice, although the CCK secretory response to trypsin inhibitor was retained. The uptake of fluorescently labeled lipoproteins in ILDR1-transfected CHO cells and release of CCK from isolated intestinal cells required a unique combination of fatty acid plus HDL. CCK secretion secondary to ILDR1 activation is associated with increased [Ca2+]i consistent with regulated hormone release. These findings demonstrate that ILDR1 regulates CCK release through a mechanism dependent on fatty acids and lipoproteins and that absorbed fatty acids regulate gastrointestinal hormone secretion. GFP positive cells from CCK-EGFP transgenic mice were isolated by FACS and the expression profile was compared with an equal number of non-fluorescent intestinal cells.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-47196", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-47196/samples/"}