ArrayExpress Uploader0humanautologous serumautologous serum + IFNa2b9708arrayexpress_sid3To explore the full extent of IFN-regulated transcriptional changes, we exposed monocytes from two healthy donors to recombinant type I...24829414E-GEOD-46921/profile/8773/arrayexpressuploader02serumJan.17, 2015Falsesle-serum-induced-transcripts-regulated-by-ifna2bE-GEOD-46921_A-AFFY-33SLE serum induced transcripts regulated by IFNa2bJan.17, 2015To explore the full extent of IFN-regulated transcriptional changes, we exposed monocytes from two healthy donors to recombinant type I IFN (IFN-α2b) in vitro. RNA was extracted at 6 hrs and the expression data was normalized to that of monocytes cultured with medium. Blood monocytes isolated from healthy volunteers were incubated with 20% autologous serum alone or in the presence of 1000 U/ml of IFNα2b (Schering Plough, Kenilworth, NJ) in 6-well plates at a concentration of 106 monocytes per well in 3 ml of media. After incubation for six hours at 37 ⁰C, cells were harvested and RNA was extracted.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46921http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46921/samples/