{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM1141218 1": {"TREATMENT": "autologous serum"}}, {"GSM1141219 1": {"TREATMENT": "autologous serum + IFNa2b"}}], "id": 9708, "ownerprofile_id": "arrayexpress_sid", "platform": 3, "summary_wrapped": "To explore the full extent of IFN-regulated transcriptional changes, we exposed monocytes from two healthy donors to recombinant type I...", "pubmed_id": 24829414, "geo_gse_id": "E-GEOD-46921", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 2, "tags": ["serum"], "lastmodified": "Jan.17, 2015", "is_default": false, "geo_gds_id": "", "slug": "sle-serum-induced-transcripts-regulated-by-ifna2b", "geo_id_plat": "E-GEOD-46921_A-AFFY-33", "name": "SLE serum induced transcripts regulated by IFNa2b", "created": "Jan.17, 2015", "summary": "To explore the full extent of IFN-regulated transcriptional changes, we exposed monocytes from two healthy donors to recombinant type I IFN (IFN-\u03b12b) in vitro. RNA was extracted at 6 hrs and the expression data was normalized to that of monocytes cultured with medium. Blood monocytes isolated from healthy volunteers were incubated with 20% autologous serum alone or in the presence of 1000 U/ml of IFN\u03b12b (Schering Plough, Kenilworth, NJ) in 6-well plates at a concentration of 106 monocytes per well in 3 ml of media. After incubation for six hours at 37 \u2070C, cells were harvested and RNA was extracted.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46921", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46921/samples/"}