{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 7294, "factors": [{"GSM113990": {"AGE": "8 weeks", "GENOTYPE": "WT", "CELL TYPE": "na\u00efve CD4+ T cells"}}, {"GSM113990": {"AGE": "8 weeks", "GENOTYPE": "WT", "CELL TYPE": "na\u00efve CD4+ T cells"}}, {"GSM113990": {"AGE": "8 weeks", "GENOTYPE": "WT", "CELL TYPE": "na\u00efve CD4+ T cells"}}, {"GSM1139904": {"AGE": "8 weeks post bone marrow transfer", "GENOTYPE": "CD45.2+MHCII-/-  bone marrow to CD45.1+ WT", "CELL TYPE": "na\ufffdve CD4+ T cells developed through the selection of only thymic MHCII"}}, {"GSM1139904": {"AGE": "8 weeks post bone marrow transfer", "GENOTYPE": "CD45.2+MHCII-/-  bone marrow to CD45.1+ WT", "CELL TYPE": "na\ufffdve CD4+ T cells developed through the selection of only thymic MHCII"}}, {"GSM1139904": {"AGE": "8 weeks post bone marrow transfer", "GENOTYPE": "CD45.2+MHCII-/-  bone marrow to CD45.1+ WT", "CELL TYPE": "na\ufffdve CD4+ T cells developed through the selection of only thymic MHCII"}}, {"GSM1139907": {"AGE": "8 weeks", "GENOTYPE": "WT", "CELL TYPE": "IMP CD4+ T cells"}}, {"GSM1139907": {"AGE": "8 weeks", "GENOTYPE": "WT", "CELL TYPE": "IMP CD4+ T cells"}}, {"GSM1139909": {"AGE": "8 weeks post bone marrow transfer", "GENOTYPE": "CD45.1+ WT bone marrow to CD45.2+MHCII-/-", "CELL TYPE": "IMP CD4+ T cells developed through the selection of hematopoietic MHCII"}}, {"GSM1139909": {"AGE": "8 weeks post bone marrow transfer", "GENOTYPE": "CD45.1+ WT bone marrow to CD45.2+MHCII-/-", "CELL TYPE": "IMP CD4+ T cells developed through the selection of hematopoietic MHCII"}}, {"GSM1139909": {"AGE": "8 weeks post bone marrow transfer", "GENOTYPE": "CD45.1+ WT bone marrow to CD45.2+MHCII-/-", "CELL TYPE": "IMP CD4+ T cells developed through the selection of hematopoietic MHCII"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Innate memory phenotype (IMP) CD4+ T cells are non-conventional \u03b1\u03b2 T cells exhibiting features of innate immune cells, characterized as...", "pubmed_id": 24610010, "geo_gse_id": "E-GEOD-46892", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 11, "tags": ["bone", "bone marrow", "cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "generating-mouse-model-with-predominant-naive-or-i", "geo_id_plat": "E-GEOD-46892_A-AFFY-45", "name": "Generating mouse model with predominant na\u00efve or innate memory phenotype CD4+ T cells", "created": "Nov.12, 2014", "summary": "Innate memory phenotype (IMP) CD4+ T cells are non-conventional \u03b1\u03b2 T cells exhibiting features of innate immune cells, characterized as CD44high and CD62Llow in periphery. It is recently reported by our group that bone marrow chimeric mice lacking thymic MHCI expression develop predominantly IMP CD8+ T cells, while those lacking hematopoietic MHCI develop predominantly na\u00efve CD8+ T cells. Here we perform hirarchical clustering analysis and found that CD4+ T cells share similar property: chimeras lacking thymic MHCII gave rise to predominantly CD4+ T cells that resemble IMP CD4+ T cells observed in WT mice, and vice versa, chimeras lacking hematopoietic MHCII had a majority of na\u00efve-like CD4+ T cells resembling na\u00efveCD4+ T cells seen in WT mice. We used microarrays to compare the global programme of gene expression to determine whether the hematopoietic MHCII selected CD4+ T cells are IMP, and whether the thymic MHCII selected CD4+ T cells are na\u00efve CD4+ T cells as observed in WT mice. Through hierarchical clustering and analysis of global gene differential expression, we determined that hematopoietic MHCII dependent IMP CD4+ T cells generated from WT bone marrow transplanted into irradiated MHCII-/- recipients, resemble IMP CD4+ T cells in WT mice, while na\u00efve CD4+ T cells generated from MHCII-/- bone marrow transplanted into irradiated WT recipients, resemble na\u00efve CD4+ T cells in WT mice. Cell Sorting was performed using a Cytopeia Influx Cell Sorter. Chimeric IMP (CD45.1+TCR\u03b2+CD4+CD44highCD62Llow) CD4+ T cells were sorted from splenocytes of CD45.1+WT\u2192CD45.2+MHCII-/- chimeras (WM IMP CD4), and chimeric na\u00efve (CD45.2+TCR\u03b2+CD4+CD44lowCD62Lhigh) CD4+ T cells were sorted from splenocytes of CD45.2+MHCII-/- \u2192 CD45.1+WT chimeras (MW na\u00efve CD4) respectively, 8 weeks post transplantation.  WT IMP (TCR\u03b2+CD4+CD44highCD62Llow) and na\u00efve (TCR\u03b2+CD4+CD44lowCD62Lhigh) CD4+ T cells were sorted from splenocytes of 8-week old WT mice.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46892", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46892/samples/"}