ArrayExpress Uploader07292Enucleated MII oocyte cytoplastsEnucleated MII oocyte cytoplastsEnucleated MII oocyte cytoplastsEnucleated MII oocyte cytoplastsMII oocytesMII oocytesMII oocytesSpindle-chromosome complexes isolated from MII oocytesSpindle-chromosome complexes isolated from MII oocytesSpindle-chromosome complexes isolated from MII oocytesarrayexpress_sid6The oocytes of many species, both invertebrate and vertebrate, contain a large collection of localized determinants in the form of...23852387E-GEOD-46875/profile/8773/arrayexpressuploader110chromosomeoocytespindleDec.12, 2014Falseassociation-of-maternal-mrna-with-the-spindle-in-mE-GEOD-46875_A-AFFY-45Association of maternal mRNA with the spindle in mouse oocytesNov.12, 2014The oocytes of many species, both invertebrate and vertebrate, contain a large collection of localized determinants in the form of proteins and translationally inactive maternal mRNAs. However, it is unknown whether mouse oocytes contain localized MmRNA determinants and what mechanisms might be responsible for their control. We collected intact MII oocytes, enucleated MII oocyte cytoplasts (with the spindle removed), and spindle-chromosome complexes which had been microsurgically removed. RNA was extracted, amplified, labeled, and applied to microarrays to determine if any MmRNA determinants were localized to the SCC. We used microarrays to determine whether maternal mRNAs in mouse oocytes are enriched in the meiotic spindlehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46875mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46875/samples/