{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "id": 7287, "factors": [{"GSM1138496 1": {}}, {"GSM1138497 1": {}}, {"GSM1138498 1": {}}, {"GSM1138499 1": {}}, {"GSM1138500 1": {}}, {"GSM1138501 1": {}}], "pop_total": 0, "platform": 6, "summary_wrapped": "Missense FBXW7 mutations are prevalent in various tumors, including T-cell acute lymphoblastic leukemia (T-ALL). To study the effects of...", "geo_gse_id": "E-GEOD-46797", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 0, "sample_count": 6, "tags": ["acute lymphoblastic leukemia", "cancer", "cell", "leukemia", "lymphoblastic leukemia", "protein", "spleen", "stem cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-c-myc-notch1-t-all-initiating", "geo_id_plat": "E-GEOD-46797_A-AFFY-45", "name": "Expression data from c-Myc+ Notch1 T-ALL initiating cells", "created": "Nov.12, 2014", "summary": "Missense FBXW7 mutations are prevalent in various tumors, including T-cell acute lymphoblastic leukemia (T-ALL). To study the effects of such lesions, we generated animals carrying regulatable Fbxw7 mutant alleles. We show here that these mutations specifically bolster cancer-initiating cell activity in collaboration with Notch1 oncogenes, but spare normal hematopoietic stem cell function. We were also able to show that FBXW7 mutations specifically affect the ubiquitylation and half-life of c-Myc protein, a key T-ALL oncogene. Using animals carrying c-Myc fusion alleles, we connected Fbxw7 function to c-Myc abundance and correlated c-Myc expression to leukemia-initiating activity. Three independent Notch1 T-ALL were derived on c-Myc-GFP background and sorted from the spleen of leukemic mice on the basis of GFP expression for RNA extraction and hybridization on Affymetrix microarrays", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46797", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46797/samples/"}