Dataset: Inducible expression of MyoD directly mediates myogenic conversion of human induced pluripotent stem cells (iPSCs) derived from Duchenne muscular dystrophy (DMD).
Genome-wide gene expression analysis of MyoD-infected DMD-specific iPSCs (GM05112-M5.1) on days 0 (untreated), day 3 and day 8 post Dox...
Genome-wide gene expression analysis of MyoD-infected DMD-specific iPSCs (GM05112-M5.1) on days 0 (untreated), day 3 and day 8 post Dox treatment, human primary myoblasts (undifferentiated and as differentiated myotubes), and undifferentiated iPSCs from healthy donors (iPSCs-1 and iPSCs-2). DMD-specific iPSCs were infected with lentivirus expressing MyoD under the control of Tet-inducible promoter and another lentivirus expressing the transactivator. To initiate myogenic differentiation, iPSCs were treated with 1µg/ml Dox. RNA was isolated 0, 3 and 8 days later and gene expression analysis was performed.
- Species:
- human
- Samples:
- 7
- Source:
- E-GEOD-46633
- PubMed:
- 24396035
- Updated:
- Dec.12, 2014
- Registered:
- Jul.11, 2014
| Sample | CELL TYPE |
|---|---|
| GSM1133460 | Human skeletal muscle myoblasts, in differentiation media (day 3) |
| GSM1133459 | Human skeletal muscle myoblasts, in expansion media |
| GSM1133458 | MyoD-infected DMD-specific iPSCs (GM05112-M5.1), day 8 post-dox addition |
| GSM1133457 | MyoD-infected DMD-specific iPSCs (GM05112-M5.1), day 3 post-dox addition |
| GSM1133456 | MyoD-infected DMD-specific iPSCs (GM05112-M5.1), untreated (feeder-free) |
| GSM1133455 | iPSCs from healthy donor (feeder-free) |
| GSM1133454 | iPSCs from healthy donor (on feeders) |