{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM1131793": {"TREATMENT": "treated with non-targeting control siRNA"}}, {"GSM1131793": {"TREATMENT": "treated with non-targeting control siRNA"}}, {"GSM113179": {"TREATMENT": "treated with non-targeting control siRNA then infected with HCV"}}, {"GSM113179": {"TREATMENT": "treated with non-targeting control siRNA then infected with HCV"}}, {"GSM1131789": {"TREATMENT": "treated with IKKalpha siRNA and subsequently infected with HCV"}}, {"GSM1131789": {"TREATMENT": "treated with IKKalpha siRNA and subsequently infected with HCV"}}, {"GSM1131787": {"TREATMENT": "treated with IKKalpha siRNA"}}, {"GSM1131787": {"TREATMENT": "treated with IKKalpha siRNA"}}], "id": 2441, "pop_total": 0, "platform": 4, "summary_wrapped": "Hepatitis C virus interacts extensively with host factors not only to establish productive infection but also to trigger unique...", "geo_gse_id": "E-GEOD-46528", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 8, "tags": ["genome", "hepatitis", "hepatitis c", "lipid", "lipid droplet"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "hepatitis-c-virus-infection-activates-a-novel-inna", "geo_id_plat": "E-GEOD-46528_A-AFFY-44", "name": "Hepatitis C Virus Infection Activates a Novel Innate Pathway Involving IKK\u03b1 in Lipogenesis and Viral Assembly", "created": "Jul.12, 2014", "summary": "Hepatitis C virus interacts extensively with host factors not only to establish productive infection but also to trigger unique pathological processes. Our recent genome-wide siRNA screen demonstrated that IKK\u03b1 is a critical host factor for HCV. Here we describe a novel NF-\u03baB-independent and kinase-mediated nuclear function of IKK\u03b1 in HCV assembly. HCV infection, through its 3\u2019-untranslated region, interacts with DDX3X to activate IKK\u03b1, which translocates to the nucleus and induces a CBP/p300-mediated transcriptional program involving SREBPs. This novel innate pathway induces lipogenic genes and enhances core-associated lipid droplet formation to facilitate viral assembly. Chemical inhibitors of IKK\u03b1 suppress HCV infection and IKK\u03b1-induced lipogenesis, offering a proof-of-concept approach for novel HCV therapeutic development. Our results show that HCV commands a novel mechanism to its advantage by exploiting intrinsic innate response and hijacking lipid metabolism, which likely contributes to a high chronicity rate and the pathological hallmark of steatosis in HCV infection. Cells were treated with either non-targeting control siRNA or siRNA against IKKalpha. After 72 h, cells were either mock infected or infected with HCV JFH-1 strain with the M.O.I. of 0.5.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46528", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46528/samples/"}