{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1126977": {"CELL TYPE SUBPOPULATION": "CD19+CD5+CD1dhigh B cells"}}, {"GSM1126978": {"CELL TYPE SUBPOPULATION": "CD19+CD5-CD1dhigh B cells"}}], "id": 7263, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "We have previously observed that regulatory B cells expressing the apoptosis-inducing surface molecule Fas ligand were enriched in the B...", "geo_gse_id": "E-GEOD-46245", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 2, "tags": ["cell", "surface"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "comparing-gene-expression-in-cd5cd1dhigh-and-cd5-c", "geo_id_plat": "E-GEOD-46245_A-AFFY-45", "name": "Comparing gene expression in CD5+CD1dhigh and CD5-CD1dhigh B cells", "created": "Nov.12, 2014", "summary": "We have previously observed that regulatory B cells expressing the apoptosis-inducing surface molecule Fas ligand were enriched in the B cell fraction denoted by a surface phenotype of CD5+CD1dhigh. To identify potential growth factors, transcriptional regulators, or surface markers that might better characterize regulatory B cells, we compared global gene expression by microarray in two B cells subsets isolated by FACS. Splenocytes from 20 DBA/1 male mice were harvested, pooled, and CD19+ B cells were isolated by positive selection using MACS. B cells were then stained for CD5 and CD1d and sorted by FACS into CD5+CD1dhigh and CD5-CD1dhigh populations. Single replicates of both CD5+CD1dhigh and CD5-CD1dhigh B cells from pooled mouse splenocytes were isolated and assay using the Affymetrix GeneChip Mouse 430 2.0 Array.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46245", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46245/samples/"}