ArrayExpress Uploader0mouseCD19+CD5+CD1dhigh B cellsCD19+CD5-CD1dhigh B cells7263arrayexpress_sid6We have previously observed that regulatory B cells expressing the apoptosis-inducing surface molecule Fas ligand were enriched in the B...E-GEOD-46245/profile/8773/arrayexpressuploader12cellsurfaceDec.12, 2014Falsecomparing-gene-expression-in-cd5cd1dhigh-and-cd5-cE-GEOD-46245_A-AFFY-45Comparing gene expression in CD5+CD1dhigh and CD5-CD1dhigh B cellsNov.12, 2014We have previously observed that regulatory B cells expressing the apoptosis-inducing surface molecule Fas ligand were enriched in the B cell fraction denoted by a surface phenotype of CD5+CD1dhigh. To identify potential growth factors, transcriptional regulators, or surface markers that might better characterize regulatory B cells, we compared global gene expression by microarray in two B cells subsets isolated by FACS. Splenocytes from 20 DBA/1 male mice were harvested, pooled, and CD19+ B cells were isolated by positive selection using MACS. B cells were then stained for CD5 and CD1d and sorted by FACS into CD5+CD1dhigh and CD5-CD1dhigh populations. Single replicates of both CD5+CD1dhigh and CD5-CD1dhigh B cells from pooled mouse splenocytes were isolated and assay using the Affymetrix GeneChip Mouse 430 2.0 Array.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46245http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-46245/samples/