Dataset: Patient-derived Human Breast Cancer Xenografts

Breast cancer research is hampered by difficulties in obtaining and studying primary human breast tissue, and by the lack of in vivo...

Registered by ArrayExpress Uploader

View Dataset

Breast cancer research is hampered by difficulties in obtaining and studying primary human breast tissue, and by the lack of in vivo preclinical models that reflect patient tumor biology accurately. To overcome these limitations, we propagated a cohort of human breast tumors grown in the epithelium-free mammary fat pad of SCID/Beige and NOD/SCID/IL2γ-receptor null (NSG) mice, under a series of transplant conditions. Both models yielded stably transplantable xenografts at comparably high rates (~23% and ~19%, respectively). Of the conditions tested, xenograft take rate was highest in the presence of a low-dose estradiol pellet. Overall, 32 stably transplantable xenograft lines were established, representing unique 25 patients. Most tumors yielding xenografts were “triple-negative” (ER-PR-HER2+) (n=19). However, we established lines from three ER-PR-HER2+ tumors, one ER+PR-HER2-, one ER+PR+HER2- and one “triple-positive” (ER+PR+HER2+) tumor. Serially passaged xenografts show biological consistency with the tumor of origin, are phenotypic stability across multiple transplant generations at the histological, transcriptomic, proteomic, and genomic levels, and show comparable treatment responses. Xenografts representing 12 patients, including two ER+ lines, showed metastasis to the mouse lung. These models thus serve as a renewable, quality-controlled tissue resource for preclinical studies investigating treatment response and metastasis. The study was designed to determine how stable patient-derived xenografts are across multiple transplant generations in mice, and to determine how closely xenografts established with pre-treatment samples cluster with xenografts established with post-treatment samples. Overall, pre-treatment and post-treatment samples derived from the same patient cluster together, and multiple transplant generations of xenografts derived from an individual patient cluster together.

Species:: human
Samples:: 44
Source:: E-GEOD-46106
Updated:: Dec.12, 2014
Registered:: Jul.12, 2014

Factors: (via ArrayExpress)

Sample	EGFR.IHC	ETHICITY	HER2.STATUS	PAM50.INTRINSIC.SUBTYPE	PATIENT.NUMBER	ER.IHC	TRANSPLANT GENERATION	CK.5.6.IHC	PR.IHC	LUNG.METASTASIS.IN.MICE	P53.IHC	PATIENT.NODAL.STATUS	CK19.IHC	ESTABLISHED.FROM.PRE.TREATMENT..I....POST.TREATMENT..P..OR.METASTATIC..M.
GSM1123825	P	C	N	H	24	N	1	N	N	N	N	N	P	M
GSM1123825	P	C	N	H	24	N	1	N	N	N	N	N	P	M
GSM1123823	N	H	P	H	23	N	1	N	N	Y	P	NR	P	M
GSM1123823	N	H	P	H	23	N	1	N	N	Y	P	NR	P	M
GSM112382	N	H	P	H	18	P	5	N	P	Y	P	P	P	P
GSM1123820	P	A	N	B	17	N	1	N	N	N	N	P	P	P
GSM1123819	P	A	N	B	16	N	1	N	N	N	N	P	P	I
GSM1123818	P	H	N	B	15	N	1	P	N	N	N	P	P	P
GSM1123817	P	H	N	B	15	N	2	P	N	Y	N	P	P	I
GSM1123816	P	C	N	B	14	N	1	P	N	Y	N	P	P	I
GSM1123815	N	H	P	H	12	N	5	N	N	Y	P	P	P	P
GSM1123814	N	H	P	H	12	N	1	N	N	Y	P	P	P	I
GSM1123813	N	H	P	H	12	N	5	N	N	Y	P	P	P	I
GSM1123812	N	H	N	H	11	N	1	N	N	N	N	N	P	P
GSM11238	N	H	N	B	10	N	5	P	N	Y	P	N	P	I
GSM1123810	N	H	N	B	10	N	1	P	N	Y	P	N	P	I
GSM1123809	P	H	N	B	9	N	5	P	N	N	N	N	N	P
GSM1123808	P	H	N	B	9	N	1	P	N	N	N	N	N	P
GSM1123807	P	H	N	B	9	N	2	P	N	N	N	N	N	I
GSM1123806	P	H	N	B	9	N	5	P	N	N	N	N	N	I
GSM1123805	N	H	N	B	8	N	1	N	N	Y	N	P	N	P
GSM1123804	N	H	N	B	8	N	5	N	N	Y	N	P	N	P
GSM1123803	P	A	N	B	7	N	1	P	N	Y	N	N	P	I
GSM1123802	P	A	N	B	7	N	5	P	N	Y	N	N	P	I
GSM112380	N	A	N	B	6	N	1	N	N	N	N	P	P	P
GSM1123800	N	A	N	B	6	N	5	N	N	N	N	P	P	I
GSM1123799	N	A	N	B	6	N	1	N	N	N	N	P	P	I
GSM1123798	P	C	N	B	5	N	5	P	N	Y	P	N	P	I
GSM1123797	P	C	N	B	5	N	1	P	N	Y	P	N	P	I
GSM1123796	P	C	P	B	4	N	5	P	N	N	P	N	P	P
GSM1123795	P	C	P	B	4	N	1	P	N	N	P	N	P	P
GSM1123796	P	C	P	B	4	N	5	P	N	N	P	N	P	P
GSM1123795	P	C	P	B	4	N	1	P	N	N	P	N	P	P
GSM1123792	P	C	N	B	3	N	3	P	N	N	P	N	P	P
GSM112379	P	C	N	B	3	N	1	P	N	N	P	N	P	P
GSM1123790	P	H	N	B	2	N	6	N	N	Y	P	N	P	P
GSM1123789	P	H	N	B	2	N	1	N	N	Y	P	N	P	P
GSM1123788	P	H	N	B	2	N	15	N	N	Y	P	N	P	P
GSM1123787	N	A	N	B	1	N	9	P	N	N	N	P	P	P
GSM1123786	N	A	N	B	1	N	1	P	N	N	N	P	P	P
GSM1123785	N	A	N	B	1	N	6	P	N	N	N	P	P	I
GSM1123784	N	A	N	B	1	N	1	P	N	N	N	P	P	I
GSM1123783	N	A	N	B	1	N	15	P	N	N	N	P	P	I
GSM1123782	N	A	N	B	1	N	11	P	N	N	N	P	P	I