Dataset: EphrinB2 Regulation by PTH and PTHrP Revealed by Molecular Profiling in Differentiating Osteoblasts
With the aim of identifying new pathways and genes regulated by PTH(1-34) and PTH-related protein 1-141 [PTHrP(1-141)] in osteoblasts,...
With the aim of identifying new pathways and genes regulated by PTH(1-34) and PTH-related protein 1-141 [PTHrP(1-141)] in osteoblasts, this study was carried out using a mouse marrow stromal cell line, Kusa 4b10, that acquires features of the osteoblastic phenotype in long-term culture conditions. After the appearance of functional PTH receptor 1 (PTHR1) in Kusa 4b10 cells, they were treated with either PTH(1-34) or PTHrP(1-141), and RNA was subjected to Affymetrix whole mouse genome array. Timecourse experiment on Kusa4b10 cell lines, treatment with PTH or PTHrP, collection at 1, 6 and 24 hours NOTE: This work has been published in 2008 (PMID 18627264); Since the original normalised data for this project is unavailable, the data has been re-normalised using a standard method for GEO submission in 2013. Therefore, the re-normalized data represented in this records may differ slightly from the original normalized data in the publication.
- Species:
- mouse
- Samples:
- 27
- Source:
- E-GEOD-45895
- PubMed:
- 18627264
- Updated:
- Dec.12, 2014
- Registered:
- Nov.12, 2014
| Sample | REPLICATE | TREATED WITH |
|---|---|---|
| GSM1119263 | 1 | None (control) for 1h |
| GSM1119264 | 2 | None (control) for 1h |
| GSM1119265 | 3 | None (control) for 1h |
| GSM1119266 | 1 | None (control) for 24h |
| GSM1119267 | 2 | None (control) for 24h |
| GSM1119268 | 3 | None (control) for 24h |
| GSM1119269 | 1 | None (control) for 6h |
| GSM1119270 | 2 | None (control) for 6h |
| GSM111927 | 3 | None (control) for 6h |
| GSM1119272 | 1 | PTH(1–34) (10 nM) for 1h |
| GSM1119273 | 2 | PTH(1–34) (10 nM) for 1h |
| GSM1119274 | 3 | PTH(1–34) (10 nM) for 1h |
| GSM1119275 | 1 | PTH(1–34) (10 nM) for 24h |
| GSM1119276 | 2 | PTH(1–34) (10 nM) for 24h |
| GSM1119277 | 3 | PTH(1–34) (10 nM) for 24h |
| GSM1119278 | 1 | PTH(1–34) (10 nM) for 6h |
| GSM1119279 | 2 | PTH(1–34) (10 nM) for 6h |
| GSM1119280 | 3 | PTH(1–34) (10 nM) for 6h |
| GSM111928 | 1 | PTHrP(1–141) (20 nM) for 1hr |
| GSM1119282 | 2 | PTHrP(1–141) (20 nM) for 1hr |
| GSM1119283 | 3 | PTHrP(1–141) (20 nM) for 1hr |
| GSM1119284 | 1 | PTHrP(1–141) (20 nM) for 24hr |
| GSM1119286 | 2 | PTHrP(1–141) (20 nM) for 24hr |
| GSM1119288 | 3 | PTHrP(1–141) (20 nM) for 24hr |
| GSM1119290 | 1 | PTHrP(1–141) (20 nM) for 6hr |
| GSM1119293 | 2 | PTHrP(1–141) (20 nM) for 6hr |
| GSM1119295 | 3 | PTHrP(1–141) (20 nM) for 6hr |