{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM1110882": {"TRANSFECTION": "control miR"}}, {"GSM1110882": {"TRANSFECTION": "control miR"}}, {"GSM1110880": {"TRANSFECTION": "pre-miR-221"}}, {"GSM1110880": {"TRANSFECTION": "pre-miR-221"}}], "id": 2089, "pop_total": 0, "platform": 4, "summary_wrapped": "MiR-221 overexpression leads to activation of apoptosis, growth arrest and reduced invasivness in PCa cells. Interaction of miR-221 with...", "geo_gse_id": "E-GEOD-45627", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 4, "tags": ["genome", "pca"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-45627_A-AFFY-44", "slug": "mir-221-mediated-gene-expression-in-human-pca-cell", "geo_gds_id": "", "name": "MiR-221 mediated gene expression in human PCa cells", "created": "Jul.11, 2014", "summary": "MiR-221 overexpression leads to activation of apoptosis, growth arrest and reduced invasivness in PCa cells. Interaction of miR-221 with potential target genes was analyzed by a genome wide expression profiling.. Regulation of selected genes and proteins identified in the gene array analysis was confirmed by Real Time RT-PCR assay (IRF1, IRF2 SOCS3, STAT1), and Western Blotting. In total, 282 genes were upregulated and 64 downregulated based on a more than 2-fold difference to untransfected PC-3 cells. Regulated genes are involved in apoptosis, hemostasis, oxidative stress response,  tumorigenesis and inflammation. We confirmed dysregulation of IRF-2 SOCS3, STAT1,IRF9. These results indicate that miR-221 overexpression might lead to activation of the JAK/STAT pathway and downregulation of miR-221 might contribute to tumorigenesis in PCa cells. pre-miR-221 transfected PC-3 cells vs unstimulated control cells - total samples analysed are 4.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-45627", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-45627/samples/"}