{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM11042": {"TREATMENT": "none", "CELL CULTURE": "IN3008"}}, {"GSM11042": {"TREATMENT": "none", "CELL CULTURE": "IN3008"}}, {"GSM1104209": {"TREATMENT": "5-Aza-dC (5 \u00ef\u00bf\u00bdmol/L)", "CELL CULTURE": "IN3008"}}, {"GSM1104209": {"TREATMENT": "5-Aza-dC (5 \u00ef\u00bf\u00bdmol/L)", "CELL CULTURE": "IN3008"}}, {"GSM1104207": {"TREATMENT": "none", "CELL CULTURE": "IN1638"}}, {"GSM1104207": {"TREATMENT": "none", "CELL CULTURE": "IN1638"}}, {"GSM1104205": {"TREATMENT": "5-Aza-dC (5 \u00ef\u00bf\u00bdmol/L)", "CELL CULTURE": "IN1638"}}, {"GSM1104205": {"TREATMENT": "5-Aza-dC (5 \u00ef\u00bf\u00bdmol/L)", "CELL CULTURE": "IN1638"}}, {"GSM1104203": {"TREATMENT": "none", "CELL CULTURE": "IN1594"}}, {"GSM1104203": {"TREATMENT": "none", "CELL CULTURE": "IN1594"}}, {"GSM110420": {"TREATMENT": "5-Aza-dC (5 \u00ef\u00bf\u00bdmol/L)", "CELL CULTURE": "IN1594"}}, {"GSM110420": {"TREATMENT": "5-Aza-dC (5 \u00ef\u00bf\u00bdmol/L)", "CELL CULTURE": "IN1594"}}], "id": 2201, "pop_total": 0, "platform": 4, "summary_wrapped": "Promoter hypermethylation and transcriptional silencing is a common epigenetic mechanism of tumour suppressor inactivation in cancer,...", "geo_gse_id": "E-GEOD-45437", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 12, "tags": ["brain", "cancer", "cell", "genome"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-45437_A-AFFY-44", "slug": "expression-data-from-paediatric-ependymoma-short-t", "geo_gds_id": "", "name": "Expression data from paediatric ependymoma short-term cell cultures", "created": "Jul.11, 2014", "summary": "Promoter hypermethylation and transcriptional silencing is a common epigenetic mechanism of tumour suppressor inactivation in cancer, including malignant brain tumours. To identify targets of epigenetic silencing mediated by CpG island methylation in paediatric ependymoma, we used a pharmacological unmasking approach through treatment with the demethylating agent 5-Aza-2\u2019-deoxycytidine followed by global expression microarray analysis. Three short-term ependymoma cell cultures were used for whole genome expression analysis following treatment with the demethylating agent 5-Aza-dC (5 \u00b5mol/L) or mock-treated with DMSO (\u22640.1% v/v) for 96-hrs.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-45437", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-45437/samples/"}