{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1104140": {"GENOTYPE": "wildtype"}}, {"GSM1104140": {"GENOTYPE": "wildtype"}}, {"GSM1104140": {"GENOTYPE": "wildtype"}}, {"GSM1104143": {"GENOTYPE": "Sox4 overexpressing leukemic"}}, {"GSM1104143": {"GENOTYPE": "Sox4 overexpressing leukemic"}}, {"GSM1104143": {"GENOTYPE": "Sox4 overexpressing leukemic"}}, {"GSM1104146": {"GENOTYPE": "K/L leukemic"}}, {"GSM1104146": {"GENOTYPE": "K/L leukemic"}}, {"GSM1104146": {"GENOTYPE": "K/L leukemic"}}], "id": 7216, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Mutation or epigenetic silencing of the transcription factor C/EBP\u03b1 is observed in ~10% of patients with acute myeloid leukemia (AML). In...", "geo_gse_id": "E-GEOD-45430", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 9, "tags": ["acute myeloid leukemia", "leukemia", "myeloid leukemia"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "sox4-is-a-key-oncogenic-target-in-cebp-mutant-acut", "geo_id_plat": "E-GEOD-45430_A-AFFY-45", "name": "Sox4 is a key oncogenic target in C/EBP\u03b1 mutant Acute Myeloid Leukemia", "created": "Nov.12, 2014", "summary": "Mutation or epigenetic silencing of the transcription factor C/EBP\u03b1 is observed in ~10% of patients with acute myeloid leukemia (AML). In both cases, a common global gene expression profile is observed, but down-stream targets relevant for leukemogenesis are not known. Here we identify Sox4 as a direct target of C/EBP\u03b1 whereby its expression is inversely correlated with C/EBP\u03b1 activity. Downregulation of Sox4 abrogated increased self-renewal of leukemic cells and restored their differentiation. Gene expression profiles of leukemia initiating cells (LICs) from both Sox4 overexpression and murine mutant C/EBP\u03b1 AML models clustered together, but differed from other types of AML. Our data demonstrate that Sox4 overexpression resulting from C/EBP\u03b1 inactivation contributes to the development of leukemias with a distinct LIC phenotype. K/L (bi-allelic Cebpa mutations) leukemic mice and Sox4 overexprssing leukemic mice were used for RNA extraction and hybridization on Affymetrix microarrays. We compared these microarray samples with the C57/BL6 wild type mice.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-45430", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-45430/samples/"}