ArrayExpress Uploader021367actinic keratosisN/AImmunocompetentmale6actinic keratosisN/AImmunosuppressedmale5actinic keratosisN/AImmunosuppressedmale4actinic keratosisN/AImmunosuppressedmale1actinic keratosisN/AImmunosuppressedmale2actinic keratosisN/AImmunosuppressedfemale11actinic keratosisN/AImmunocompetentmale9actinic keratosisN/AImmunosuppressedmale10actinic keratosisN/AImmunosuppressedmale8actinic keratosisN/AImmunocompetentmale29Cutaneous SCCModerately-poorly differentiatedImmunosuppressedmale20Cutaneous SCCPoorly differentiatedImmunosuppressedmale19Cutaneous SCCPoorly differentiatedImmunosuppressedmale18Cutaneous SCCModerately-poorly differentiatedImmunosuppressedfemale13Cutaneous SCCModerately-poorly differentiatedImmunosuppressedfemale12Cutaneous SCCModerately-poorly differentiatedImmunocompetentfemale28Cutaneous SCCModerately differentiatedImmunocompetentmale27Cutaneous SCCModerately differentiatedImmunosuppressedmale26Cutaneous SCCModerately differentiatedImmunosuppressedmale25Cutaneous SCCModerately differentiatedImmunosuppressedmale24Cutaneous SCCWell-moderately differentiatedImmunosuppressedmale21Cutaneous SCCModerately differentiatedImmunosuppressedfemale17Cutaneous SCCModerately differentiatedImmunocompetentmale8Cutaneous SCCModerately differentiatedImmunocompetentmale6Cutaneous SCCModerately differentiatedImmunosuppressedmale31Cutaneous SCCWell differentiatedImmunosuppressedmale30Cutaneous SCCWell differentiatedImmunocompetentmale23Cutaneous SCCWell differentiatedImmunosuppressedfemale22Cutaneous SCCWell differentiatedImmunosuppressedmale16Cutaneous SCCWell differentiatedImmunosuppressedmale15Cutaneous SCCWell differentiatedImmunocompetentmale14Cutaneous SCCWell differentiatedImmunocompetentmale11Cutaneous SCCWell differentiatedImmunocompetentmale10Cutaneous SCCWell differentiatedImmunosuprresedmale9Cutaneous SCCWell differentiatedImmunosuppressedmale5Cutaneous SCCWell differentiatedImmunosuppressedmale4Cutaneous SCCWell differentiatedImmunosuppressedmale3Cutaneous SCCWell differentiatedImmunocompetentmale2Cutaneous SCCWell differentiatedImmunosuppressedfemale1Cutaneous SCCWell differentiatedImmunosuppressedmalearrayexpress_sid4Cutaneous squamous cell carcinoma (cSCC) is one of the most common malignancies in fair skinned populations worldwide and its incidence...24335922E-GEOD-45216/profile/8773/arrayexpressuploader540carcinomacellinvasive carcinomasquamoussquamous cellsquamous cell carcinomaDec.12, 2014Falsekey-differences-identified-between-actinic-keratosE-GEOD-45216_A-AFFY-44Key differences identified between actinic keratosis and cutaneous squamous cell carcinoma by transcriptome profilingJul.11, 2014Cutaneous squamous cell carcinoma (cSCC) is one of the most common malignancies in fair skinned populations worldwide and its incidence is increasing. Despite previous observations of multiple genetic abnormalities in cSCC, the oncogenic process remains elusive. The purpose of this study was to investigate the transcriptomes of cSCC and actinic keratoses (AK), to elucidate key differences between precursor AK lesions and invasive carcinoma. This study identified 196 genes that are differentially expressed between AK and cSCC, with enrichment for processes including epidermal differentiation, cell migration, cell cycle regulation and metabolism. Gene set enrichment analysis highlighted a key role for the MAPK pathway in the transition from AK to cSCC. Furthermore, the differentiation status of the tumor influenced the gene expression profile, which may have implications for drug sensitivity and response in clinical trials. These data indicate that progression to cSCC is associated with a complex pattern of molecular changes. We have identified relevant pathways involved in this process, in particular that the MAPK pathway may be pivotal to the transition from AK and may represent a potential therapeutic target. RNA was extracted by laser capture microdissection from 10 AK and 30 cSCC, for analysis using the Affymetrix HG U133 Plus 2.0 microarrays. The cSCC samples included a range of histological diagnoses (well differentiated through to poorly differentiatied) from both immunocompetent and immunosuppressed patients.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-45216humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-45216/samples/