{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "human", "factors": [{"GSM1098984": {"TREATMENT": "450mg/kg AGI-5198"}}, {"GSM1098984": {"TREATMENT": "450mg/kg AGI-5198"}}, {"GSM1098984": {"TREATMENT": "450mg/kg AGI-5198"}}, {"GSM1098984": {"TREATMENT": "450mg/kg AGI-5198"}}, {"GSM1098984": {"TREATMENT": "450mg/kg AGI-5198"}}, {"GSM1098984": {"TREATMENT": "450mg/kg AGI-5198"}}, {"GSM1098984": {"TREATMENT": "450mg/kg AGI-5198"}}, {"GSM1098977": {"TREATMENT": "150mg/kg AGI-5198"}}, {"GSM1098977": {"TREATMENT": "150mg/kg AGI-5198"}}, {"GSM1098977": {"TREATMENT": "150mg/kg AGI-5198"}}, {"GSM1098977": {"TREATMENT": "150mg/kg AGI-5198"}}, {"GSM1098977": {"TREATMENT": "150mg/kg AGI-5198"}}, {"GSM1098977": {"TREATMENT": "150mg/kg AGI-5198"}}, {"GSM1098977": {"TREATMENT": "150mg/kg AGI-5198"}}, {"GSM1098977": {"TREATMENT": "150mg/kg AGI-5198"}}, {"GSM1098969": {"TREATMENT": "vehicle"}}, {"GSM1098969": {"TREATMENT": "vehicle"}}, {"GSM1098969": {"TREATMENT": "vehicle"}}, {"GSM1098969": {"TREATMENT": "vehicle"}}, {"GSM1098969": {"TREATMENT": "vehicle"}}, {"GSM1098969": {"TREATMENT": "vehicle"}}, {"GSM1098969": {"TREATMENT": "vehicle"}}, {"GSM1098969": {"TREATMENT": "vehicle"}}, {"GSM1098969": {"TREATMENT": "vehicle"}}, {"GSM1098960": {"TREATMENT": "AGI-5198"}}, {"GSM1098969": {"TREATMENT": "vehicle"}}, {"GSM1098960": {"TREATMENT": "AGI-5198"}}, {"GSM1098969": {"TREATMENT": "vehicle"}}, {"GSM1098969": {"TREATMENT": "vehicle"}}, {"GSM1098960": {"TREATMENT": "AGI-5198"}}, {"GSM1098960": {"TREATMENT": "AGI-5198"}}, {"GSM1098960": {"TREATMENT": "AGI-5198"}}, {"GSM1098969": {"TREATMENT": "vehicle"}}], "id": 2477, "pop_total": 0, "platform": 4, "summary_wrapped": "The recent discovery of mutations in metabolic enzymes has rekindled interest in harnessing the altered metabolism of cancer cells for...", "geo_gse_id": "E-GEOD-45197", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 33, "tags": ["cancer", "genome", "glioma", "histone"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "an-inhibitor-of-mutant-idh1-delays-growth-and-prom", "geo_id_plat": "E-GEOD-45197_A-AFFY-44", "name": "An Inhibitor of Mutant IDH1 Delays Growth and Promotes Differentiation of Glioma Cells Expression data for Xenograft Samples", "created": "Jul.12, 2014", "summary": "The recent discovery of mutations in metabolic enzymes has rekindled interest in harnessing the altered metabolism of cancer cells for cancer therapy. One potential drug target is isocitrate dehydrogenase 1 (IDH1) which is mutated in multiple human cancers. Here, we examine the role of mutant IDH1 in fully transformed cells with endogenous IDH1 mutations. A selective R132H-IDH1 inhibitor (AGI-5198) identified through a high-throughput screen dose-dependently blocked the ability of the mutant enzyme (mIDH1) to produce R-2-hydroxyglutarate (R-2HG). Under conditions of near complete R-2HG inhibition, the mIDH1 inhibitor induced demethylation of histone H3K9M3 and expression of genes associated with gliogenic differentiation. Blockade of mIDH1 impaired the growth of IDH1-mutant - but not IDH1-wildtype \u2013 glioma cells without appreciable changes in genome wide DNA methylation. These data suggest that mIDH1 may promote glioma growth through mechanisms beyond its well-characterized epigenetic effects. Two xenograft experiments were carried out, one with treatment cohorts of vehicle and 450mg/kg, and the other with vehicle, 150mg/kg/day, and 450mg/kg/day. After the indicated time tumors were harvested and total RNA was extracted and analyzed by the Affymetrix U133 plus 2 array.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-45197", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-45197/samples/"}