ArrayExpress Uploader0humanhighproliferating B cellsyeslowproliferating B cellsyeslowundivided B cellsnohighproliferating B cellsyeslowproliferating B cellsyeslowundivided B cellsnohighproliferating B cellsyeslowproliferating B cellsyeslowundivided B cellsnohighproliferating B cellsyeslowproliferating B cellsyeslowundivided B cellsnohighproliferating B cellsyeslowproliferating B cellsyeslowundivided B cellsnohighproliferating B cellsyeslowproliferating B cellsyeslowundivided B cellsno2511arrayexpress_sid4During the human B cell (Bc) recall response, rapid cell division results in multiple Bc subpopulations. The TLR-9 agonist CpG...E-GEOD-45113/profile/8773/arrayexpressuploader318cellcytokinegenomeplasmablastsurfaceDec.12, 2014Falsegene-expression-of-cpg-activated-memory-b-cellsE-GEOD-45113_A-AFFY-44Gene expression of CpG-activated memory B cellsJul.12, 2014During the human B cell (Bc) recall response, rapid cell division results in multiple Bc subpopulations. The TLR-9 agonist CpG oligodeoxynucleotide, combined with cytokines, causes Bc activation and division in vitro and increased CD27 surface expression in a sub-population of Bc. We hypothesized that the proliferating CD27lo subpopulation, which has a lower frequency of antibody-secreting cells (ASC) than CD27hi plasmablasts, provides alternative functions such as cytokine secretion, costimulation, or antigen presentation. We performed genome-wide transcriptional analysis of CpG activated Bc sorted into undivided, proliferating CD27lo and proliferating CD27hi subpopulations. Our data supported an alternative hypothesis, that CD27lo cells are a transient pre-plasmablast population, expressing genes associated with Bc receptor editing. Undivided cells had an active transcriptional program of non-ASC B cell functions, including cytokine secretion and costimulation, suggesting a link between innate and adaptive Bc responses. Transcriptome analysis suggested a gene regulatory network for CD27lo and CD27hi Bc differentiation. 18 B cell samples from 6 subjects. From each subject, 3 in vitro B cell populations were isolated: undivided cells, proliferating cells with low CD27 (marker of antibody secretion), proliferating cells with high CD27http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-45113http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-45113/samples/