{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM1097014": {"CD27": "high", "CELL TYPE": "proliferating B cells", "DIVIDED IN VITRO": "yes"}}, {"GSM1097013": {"CD27": "low", "CELL TYPE": "proliferating B cells", "DIVIDED IN VITRO": "yes"}}, {"GSM1097012": {"CD27": "low", "CELL TYPE": "undivided B cells", "DIVIDED IN VITRO": "no"}}, {"GSM1097014": {"CD27": "high", "CELL TYPE": "proliferating B cells", "DIVIDED IN VITRO": "yes"}}, {"GSM1097013": {"CD27": "low", "CELL TYPE": "proliferating B cells", "DIVIDED IN VITRO": "yes"}}, {"GSM1097012": {"CD27": "low", "CELL TYPE": "undivided B cells", "DIVIDED IN VITRO": "no"}}, {"GSM1097014": {"CD27": "high", "CELL TYPE": "proliferating B cells", "DIVIDED IN VITRO": "yes"}}, {"GSM1097013": {"CD27": "low", "CELL TYPE": "proliferating B cells", "DIVIDED IN VITRO": "yes"}}, {"GSM1097012": {"CD27": "low", "CELL TYPE": "undivided B cells", "DIVIDED IN VITRO": "no"}}, {"GSM1097014": {"CD27": "high", "CELL TYPE": "proliferating B cells", "DIVIDED IN VITRO": "yes"}}, {"GSM1097013": {"CD27": "low", "CELL TYPE": "proliferating B cells", "DIVIDED IN VITRO": "yes"}}, {"GSM1097012": {"CD27": "low", "CELL TYPE": "undivided B cells", "DIVIDED IN VITRO": "no"}}, {"GSM1097014": {"CD27": "high", "CELL TYPE": "proliferating B cells", "DIVIDED IN VITRO": "yes"}}, {"GSM1097013": {"CD27": "low", "CELL TYPE": "proliferating B cells", "DIVIDED IN VITRO": "yes"}}, {"GSM1097012": {"CD27": "low", "CELL TYPE": "undivided B cells", "DIVIDED IN VITRO": "no"}}, {"GSM1097014": {"CD27": "high", "CELL TYPE": "proliferating B cells", "DIVIDED IN VITRO": "yes"}}, {"GSM1097013": {"CD27": "low", "CELL TYPE": "proliferating B cells", "DIVIDED IN VITRO": "yes"}}, {"GSM1097012": {"CD27": "low", "CELL TYPE": "undivided B cells", "DIVIDED IN VITRO": "no"}}], "id": 2511, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "During the human B cell (Bc) recall response, rapid cell division results in multiple Bc subpopulations. The TLR-9 agonist CpG...", "geo_gse_id": "E-GEOD-45113", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 18, "tags": ["cell", "cytokine", "genome", "plasmablast", "surface"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "gene-expression-of-cpg-activated-memory-b-cells", "geo_id_plat": "E-GEOD-45113_A-AFFY-44", "name": "Gene expression of CpG-activated memory B cells", "created": "Jul.12, 2014", "summary": "During the human B cell (Bc) recall response, rapid cell division results in multiple Bc subpopulations. The TLR-9 agonist CpG oligodeoxynucleotide, combined with cytokines, causes Bc activation and division in vitro and increased CD27 surface expression in a sub-population of Bc. We hypothesized that the proliferating CD27lo subpopulation, which has a lower frequency of antibody-secreting cells (ASC) than CD27hi plasmablasts, provides alternative functions such as cytokine secretion, costimulation, or antigen presentation. We performed genome-wide transcriptional analysis of CpG activated Bc sorted into undivided, proliferating CD27lo and proliferating CD27hi subpopulations. Our data supported an alternative hypothesis, that CD27lo cells are a transient pre-plasmablast population, expressing genes associated with Bc receptor editing. Undivided cells had an active transcriptional program of non-ASC B cell functions, including cytokine secretion and costimulation, suggesting a link between innate and adaptive Bc responses. Transcriptome analysis suggested a gene regulatory network for CD27lo and CD27hi Bc differentiation. 18 B cell samples from 6 subjects. From each subject, 3 in vitro B cell populations were isolated: undivided cells, proliferating cells with low CD27 (marker of antibody secretion), proliferating cells with high CD27", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-45113", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-45113/samples/"}