ArrayExpress Uploaderarrayexpress_sid7196CTLCTLCTLIDH1wtIDH1wtIDH1wtIDH1mutIDH1mutIDH1mut06Mutations in the enzymes IDH1 and IDH2 have been identified in a wide variety of tumors like glioma, chondrosarcoma, thyroid cancer,...23954893E-GEOD-45019/profile/8773/arrayexpressuploader19acute myeloid leukemiabonebone marrowcancerchondrosarcomagliomaleukemialymphomamelanomamyeloid leukemiathyroid cancerDec.12, 2014Falsegene-expression-changes-induced-by-overexpressio-3E-GEOD-45019_A-AFFY-45Gene expression changes induced by overexpression of IDH1mut in the sorted mouse bone marrow cellsNov.12, 2014Mutations in the enzymes IDH1 and IDH2 have been identified in a wide variety of tumors like glioma, chondrosarcoma, thyroid cancer, lymphoma, melanoma, and in acute myeloid leukemia. Mutated IDH1/2 produces the metabolite 2-hydroxyglutarate (2HG), which interferes with epigenetic regulation of gene expression, and thus may promote tumorigenesis. HoxA9 immortalized bone marrow cells from C57BJ/6 mice were transduced with either empty vector or pSF91-IDH1wt-IRES-EGFP, or pSF91-IDH1mut-IRES-GFP and transplanted in irradiated recipient mice. Four weeks after transplantation, GFP+ cells were sorted from mouse bone marrow, from which total RNA was extracted and subjected to microarray analysis.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-45019mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-45019/samples/