ArrayExpress Uploader0humanNormal cerebellum controlPilocytic astrocytoma: N/A26N/AmalecerebellumNormal cerebellum controlPilocytic astrocytoma: N/AadultN/AmalecerebellumNormal cerebellum controlPilocytic astrocytoma: N/AadultN/AmalecerebellumNormal cerebellum controlPilocytic astrocytoma: N/A24N/AmalecerebellumNormal cerebellum controlPilocytic astrocytoma: N/AFetalN/AfemalecerebellumNormal cerebellum controlPilocytic astrocytoma: N/AFetalN/AmalecerebellumNormal cerebellum controlPilocytic astrocytoma: N/AFetalN/AmalecerebellumNormal cerebellum controlPilocytic astrocytoma: N/AFetalN/AfemalecerebellumNormal cerebellum controlPilocytic astrocytoma: N/AFetalN/AfemalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:9121malediencephalonPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:982femalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: BRAF V600E31femaleoptic nervePilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:932malecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_15:926NAfemalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:992femalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:9112femalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:932femalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: NF112NAfemaleLeft frontal lobePilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:916NAmalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:920NAfemalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:942malecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:926NAfemalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:1125NAmalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:991maleFourth VentriclePilocytic AstrocytomaPilocytic astrocytoma: None identified142femalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:920NAfemalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:962femalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:942malecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_15:981femalecerebral hemispherePilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_15:971maleBrainstemPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:952malecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:962malecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:942malecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:922malecerebellumPilocytic AstrocytomaPilocytic astrocytoma: BRAF V600E31femalediencephalonPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:9102malecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KRAS E63K112femalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:9222femalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:9121femalediencephalonPilocytic AstrocytomaPilocytic astrocytoma: BRAF ins598T172femalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: NF1; KIAA1549:BRAF fusion_15:9; BRAF V600E31femalediencephalonPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:11112malecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_15:9192malecerebellumPilocytic AstrocytomaPilocytic astrocytoma: NF1; KIAA1549:BRAF fusion_breakpoint not determined31malediencephalonPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:9132malecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_15:9102malecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_breakpoint not determined21femalediencephalonPilocytic AstrocytomaPilocytic astrocytoma: BRAF V600E141malecerebral hemispherePilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:9162malecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:942malecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_15:9112femalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_15:922malecerebellumPilocytic AstrocytomaPilocytic astrocytoma: FAM131B:BRAF fusion161malecerebral hemispherePilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:952femalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:942femalecerebellumPilocytic AstrocytomaPilocytic astrocytoma: None identified17NAmalecerebral hemispherePilocytic AstrocytomaPilocytic astrocytoma: KIAA1549:BRAF fusion_16:992malecerebellumPilocytic AstrocytomaPilocytic astrocytoma: None identified21NAmalecerebellum2402arrayexpress_sid4Pilocytic astrocytomas (PA) are the most common brain tumor in pediatric patients and can cause significant morbidity, including chronic...23660940E-GEOD-44971/profile/8773/arrayexpressuploader658braincerebellumproteinDec.12, 2014FalseE-GEOD-44971_A-AFFY-44gene-expression-data-from-pilocytic-astrocytoma-tuGene expression data from pilocytic astrocytoma tumour samples and normal cerebellum controlsJul.12, 2014Pilocytic astrocytomas (PA) are the most common brain tumor in pediatric patients and can cause significant morbidity, including chronic neurological deficiencies. They are characterized by activating alterations in the mitogen-activated protein kinase (MAPK) pathway, but little else is known about their development. To further define their molecular development, we analysed the global DNA methylation profiles of 61 PAs and 6 normal cerebellum samples and integrated this data with transcriptome profiling. These data revealed two subgroups of PA that separate according to tumor location (infratentorial versus supratentorial), and identified key neural developmental genes that are differentially methylated between the two groups. Significant expression differences were identified for the majority of differentially methylated genes, and these were unexpectedly associated with a strong positive correlation between methylation and expression. We also identified a large number of differentially methylated/expressed genes between cerebellar PAs and normal cerebellum, which included additional developmental genes. Total RNA from 49 PA tumour samples and 9 normal cerebellum samples (from commercial sources) were hybridised to the Affymetrix HG U133 Plus 2.0 microarrays.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-44971http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-44971/samples/