{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM109487": {"ORGANISM PART": "Normal cerebellum control", "MAPK HIT": "Pilocytic astrocytoma: N/A", "AGE AT DIAGNOSIS YEARS": "26", "DNA METHYLATION SUBGROUP": "N/A", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094870": {"ORGANISM PART": "Normal cerebellum control", "MAPK HIT": "Pilocytic astrocytoma: N/A", "AGE AT DIAGNOSIS YEARS": "adult", "DNA METHYLATION SUBGROUP": "N/A", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094870": {"ORGANISM PART": "Normal cerebellum control", "MAPK HIT": "Pilocytic astrocytoma: N/A", "AGE AT DIAGNOSIS YEARS": "adult", "DNA METHYLATION SUBGROUP": "N/A", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094868": {"ORGANISM PART": "Normal cerebellum control", "MAPK HIT": "Pilocytic astrocytoma: N/A", "AGE AT DIAGNOSIS YEARS": "24", "DNA METHYLATION SUBGROUP": "N/A", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094867": {"ORGANISM PART": "Normal cerebellum control", "MAPK HIT": "Pilocytic astrocytoma: N/A", "AGE AT DIAGNOSIS YEARS": "Fetal", "DNA METHYLATION SUBGROUP": "N/A", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094866": {"ORGANISM PART": "Normal cerebellum control", "MAPK HIT": "Pilocytic astrocytoma: N/A", "AGE AT DIAGNOSIS YEARS": "Fetal", "DNA METHYLATION SUBGROUP": "N/A", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094866": {"ORGANISM PART": "Normal cerebellum control", "MAPK HIT": "Pilocytic astrocytoma: N/A", "AGE AT DIAGNOSIS YEARS": "Fetal", "DNA METHYLATION SUBGROUP": "N/A", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094867": {"ORGANISM PART": "Normal cerebellum control", "MAPK HIT": "Pilocytic astrocytoma: N/A", "AGE AT DIAGNOSIS YEARS": "Fetal", "DNA METHYLATION SUBGROUP": "N/A", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094867": {"ORGANISM PART": "Normal cerebellum control", "MAPK HIT": "Pilocytic astrocytoma: N/A", "AGE AT DIAGNOSIS YEARS": "Fetal", "DNA METHYLATION SUBGROUP": "N/A", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094862": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "12", "DNA METHYLATION SUBGROUP": "1", "SEX": "male", "BRAIN LOCATION": "diencephalon"}}, {"GSM109486": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "8", "DNA METHYLATION SUBGROUP": "2", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094860": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: BRAF V600E", "AGE AT DIAGNOSIS YEARS": "3", "DNA METHYLATION SUBGROUP": "1", "SEX": "female", "BRAIN LOCATION": "optic nerve"}}, {"GSM1094859": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "3", "DNA METHYLATION SUBGROUP": "2", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094858": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_15:9", "AGE AT DIAGNOSIS YEARS": "26", "DNA METHYLATION SUBGROUP": "NA", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094857": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "9", "DNA METHYLATION SUBGROUP": "2", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094856": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "11", "DNA METHYLATION SUBGROUP": "2", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094855": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "3", "DNA METHYLATION SUBGROUP": "2", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094854": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: NF1", "AGE AT DIAGNOSIS YEARS": "12", "DNA METHYLATION SUBGROUP": "NA", "SEX": "female", "BRAIN LOCATION": "Left frontal lobe"}}, {"GSM1094853": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "16", "DNA METHYLATION SUBGROUP": "NA", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094852": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "20", "DNA METHYLATION SUBGROUP": "NA", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM109485": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "4", "DNA METHYLATION SUBGROUP": "2", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094850": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "26", "DNA METHYLATION SUBGROUP": "NA", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094849": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:11", "AGE AT DIAGNOSIS YEARS": "25", "DNA METHYLATION SUBGROUP": "NA", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094848": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "9", "DNA METHYLATION SUBGROUP": "1", "SEX": "male", "BRAIN LOCATION": "Fourth Ventricle"}}, {"GSM1094847": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: None identified", "AGE AT DIAGNOSIS YEARS": "14", "DNA METHYLATION SUBGROUP": "2", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094852": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "20", "DNA METHYLATION SUBGROUP": "NA", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094845": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "6", "DNA METHYLATION SUBGROUP": "2", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM109485": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "4", "DNA METHYLATION SUBGROUP": "2", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094843": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_15:9", "AGE AT DIAGNOSIS YEARS": "8", "DNA METHYLATION SUBGROUP": "1", "SEX": "female", "BRAIN LOCATION": "cerebral hemisphere"}}, {"GSM1094842": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_15:9", "AGE AT DIAGNOSIS YEARS": "7", "DNA METHYLATION SUBGROUP": "1", "SEX": "male", "BRAIN LOCATION": "Brainstem"}}, {"GSM109484": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "5", "DNA METHYLATION SUBGROUP": "2", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094840": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "6", "DNA METHYLATION SUBGROUP": "2", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM109485": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "4", "DNA METHYLATION SUBGROUP": "2", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094838": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "2", "DNA METHYLATION SUBGROUP": "2", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094837": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: BRAF V600E", "AGE AT DIAGNOSIS YEARS": "3", "DNA METHYLATION SUBGROUP": "1", "SEX": "female", "BRAIN LOCATION": "diencephalon"}}, {"GSM1094836": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "10", "DNA METHYLATION SUBGROUP": "2", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094835": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KRAS E63K", "AGE AT DIAGNOSIS YEARS": "11", "DNA METHYLATION SUBGROUP": "2", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094834": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "22", "DNA METHYLATION SUBGROUP": "2", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094833": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "12", "DNA METHYLATION SUBGROUP": "1", "SEX": "female", "BRAIN LOCATION": "diencephalon"}}, {"GSM1094832": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: BRAF ins598T", "AGE AT DIAGNOSIS YEARS": "17", "DNA METHYLATION SUBGROUP": "2", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM109483": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: NF1; KIAA1549:BRAF fusion_15:9; BRAF V600E", "AGE AT DIAGNOSIS YEARS": "3", "DNA METHYLATION SUBGROUP": "1", "SEX": "female", "BRAIN LOCATION": "diencephalon"}}, {"GSM1094830": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:11", "AGE AT DIAGNOSIS YEARS": "11", "DNA METHYLATION SUBGROUP": "2", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094829": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_15:9", "AGE AT DIAGNOSIS YEARS": "19", "DNA METHYLATION SUBGROUP": "2", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094828": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: NF1; KIAA1549:BRAF fusion_breakpoint not determined", "AGE AT DIAGNOSIS YEARS": "3", "DNA METHYLATION SUBGROUP": "1", "SEX": "male", "BRAIN LOCATION": "diencephalon"}}, {"GSM1094827": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "13", "DNA METHYLATION SUBGROUP": "2", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094826": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_15:9", "AGE AT DIAGNOSIS YEARS": "10", "DNA METHYLATION SUBGROUP": "2", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094825": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_breakpoint not determined", "AGE AT DIAGNOSIS YEARS": "2", "DNA METHYLATION SUBGROUP": "1", "SEX": "female", "BRAIN LOCATION": "diencephalon"}}, {"GSM1094824": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: BRAF V600E", "AGE AT DIAGNOSIS YEARS": "14", "DNA METHYLATION SUBGROUP": "1", "SEX": "male", "BRAIN LOCATION": "cerebral hemisphere"}}, {"GSM1094823": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "16", "DNA METHYLATION SUBGROUP": "2", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM109485": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "4", "DNA METHYLATION SUBGROUP": "2", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM109482": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_15:9", "AGE AT DIAGNOSIS YEARS": "11", "DNA METHYLATION SUBGROUP": "2", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094820": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_15:9", "AGE AT DIAGNOSIS YEARS": "2", "DNA METHYLATION SUBGROUP": "2", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094819": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: FAM131B:BRAF fusion", "AGE AT DIAGNOSIS YEARS": "16", "DNA METHYLATION SUBGROUP": "1", "SEX": "male", "BRAIN LOCATION": "cerebral hemisphere"}}, {"GSM1094818": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "5", "DNA METHYLATION SUBGROUP": "2", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094817": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "4", "DNA METHYLATION SUBGROUP": "2", "SEX": "female", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094816": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: None identified", "AGE AT DIAGNOSIS YEARS": "17", "DNA METHYLATION SUBGROUP": "NA", "SEX": "male", "BRAIN LOCATION": "cerebral hemisphere"}}, {"GSM1094815": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: KIAA1549:BRAF fusion_16:9", "AGE AT DIAGNOSIS YEARS": "9", "DNA METHYLATION SUBGROUP": "2", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}, {"GSM1094814": {"ORGANISM PART": "Pilocytic Astrocytoma", "MAPK HIT": "Pilocytic astrocytoma: None identified", "AGE AT DIAGNOSIS YEARS": "21", "DNA METHYLATION SUBGROUP": "NA", "SEX": "male", "BRAIN LOCATION": "cerebellum"}}], "id": 2402, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "Pilocytic astrocytomas (PA) are the most common brain tumor in pediatric patients and can cause significant morbidity, including chronic...", "pubmed_id": 23660940, "geo_gse_id": "E-GEOD-44971", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 6, "sample_count": 58, "tags": ["brain", "cerebellum", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-44971_A-AFFY-44", "slug": "gene-expression-data-from-pilocytic-astrocytoma-tu", "geo_gds_id": "", "name": "Gene expression data from pilocytic astrocytoma tumour samples and normal cerebellum controls", "created": "Jul.12, 2014", "summary": "Pilocytic astrocytomas (PA) are the most common brain tumor in pediatric patients and can cause significant morbidity, including chronic neurological deficiencies. They are characterized by activating alterations in the mitogen-activated protein kinase (MAPK) pathway, but little else is known about their development. To further define their molecular development, we analysed the global DNA methylation profiles of 61 PAs and 6 normal cerebellum samples and integrated this data with transcriptome profiling. These data revealed two subgroups of PA that separate according to tumor location (infratentorial versus supratentorial), and identified key neural developmental genes that are differentially methylated between the two groups. Significant expression differences were identified for the majority of differentially methylated genes, and these were unexpectedly associated with a strong positive correlation between methylation and expression. We also identified a large number of differentially methylated/expressed genes between cerebellar PAs and normal cerebellum, which included additional developmental genes. Total RNA from 49 PA tumour samples and 9 normal cerebellum samples (from commercial sources) were hybridised to the Affymetrix HG U133 Plus 2.0 microarrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-44971", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-44971/samples/"}