ArrayExpress Uploaderarrayexpress_sidhumanpartially insensitiveW741C/T877A Mut overexpression10uM MDV3100partially insensitiveW741C/T877A Mut overexpression10uM MDV3100partially insensitiveF876L/T877A Mut overexpression10uM MDV3100partially insensitiveF876L/T877A Mut overexpression10uM MDV3100sensitiveT877A WT overexpression10uM MDV3100sensitiveT877A WT overexpression10uM MDV3100sensitiveVec control10uM MDV3100sensitiveVec control10uM MDV3100partially insensitiveW741C/T877A Mut overexpression0.1% DMSOpartially insensitiveW741C/T877A Mut overexpression0.1% DMSOpartially insensitiveF876L/T877A Mut overexpression0.1% DMSOpartially insensitiveF876L/T877A Mut overexpression0.1% DMSOsensitiveT877A WT overexpression0.1% DMSOsensitiveT877A WT overexpression0.1% DMSOsensitiveVec control0.1% DMSOsensitiveVec control0.1% DMSO227204Genetically engineered LNCaPs overexpressing various AR alleles were treated with 0.1% DMSO or 10uM MDV3100 for 24h prior to collection...E-GEOD-44924/profile/8773/arrayexpressuploader316Dec.12, 2014Falsetesting-rescue-of-ar-signaling-by-ectopic-expressiE-GEOD-44924_A-AFFY-44Testing rescue of AR signaling by ectopic expression of mutant AR allele in the presence of MDV-3100Jul.11, 2014Genetically engineered LNCaPs overexpressing various AR alleles were treated with 0.1% DMSO or 10uM MDV3100 for 24h prior to collection This experiment is designed to see if expressing the F876L/T877A mutant AR can rescue AR signaling in the presence of MDV3100 Engineered lines were seeded in 6-well plates for 3d with 100ng/ml doxycycline prior to treatment with 0.1% DMSO or 10uM MDV3100 for 24hhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-44924http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-44924/samples/