ArrayExpress Uploader0mouseWTsalineWTsalineWTsalineWTsalineWT20 mg/kg LPS isolated from E. coliWT20 mg/kg LPS isolated from E. coliWT20 mg/kg LPS isolated from E. coliWT20 mg/kg LPS isolated from E. coliA2AR KOsalineA2AR KOsalineA2AR KOsalineA2AR KOsalineA2AR KO20 mg/kg LPS isolated from E. coliA2AR KO20 mg/kg LPS isolated from E. coliA2AR KO20 mg/kg LPS isolated from E. coliA2AR KO20 mg/kg LPS isolated from E. coli7183arrayexpress_sid6Analysis of venticular myocardium from adenosine 2A receptors (A2AR) knockouts following LPS stimulation. Results provide insight into...E-GEOD-44363/profile/8773/arrayexpressuploader216cellheartlipopolysaccharidemyocarditismyocardiumDec.12, 2014Falsetranscriptomic-modulation-by-adenosine-2a-receptorE-GEOD-44363_A-AFFY-45Transcriptomic modulation by adenosine 2A receptors in normal and endotoxemic myocardiumNov.12, 2014Analysis of venticular myocardium from adenosine 2A receptors (A2AR) knockouts following LPS stimulation. Results provide insight into the molecular components of A2AR mediated protection, but also reveal pathogenetic components of endotoxemic myocarditis as a result of LPS exposure. These findings demonstrate that intrinsic A2AR activity exerts limited transcriptional effects in unstressed heart, modifying G-coupled cAMP/PKA signal paths. LPS-dependent injury and dysfunction is associated with profound up-regulation of inflammatory/immune processes, fibrotic and cell death paths, and NF-kB, Erk/MAPK and JAK/Stat signaling, with shifts in multiple determinants of cardiac contraction and survival. Intrinsic A2AR activity modulates key aspects of these inflammatory responses, involving MAPK, JAK/Stat and NF-kB signaling Total RNA obtained from adenosine 2A receptor knockout or wild-type murine ventricular myocardium that were treated for 24 hours with either saline or lipopolysaccharide (n=4/group).http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-44363http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-44363/samples/