{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1081335": {"CELL TYPE": "PGK12.1 ES cell line"}}, {"GSM1081336": {"CELL TYPE": "differentiated PGK12.1 cells"}}], "id": 7173, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Affymetrix 430 2.0 mouse arrays were used for expression analyses in undifferentiated and differentiated PGK12.1 ES cells. We found that...", "geo_gse_id": "E-GEOD-44251", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 2, "tags": ["autosome", "body", "chromosome", "x chromosome"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-44251_A-AFFY-45", "slug": "expression-data-from-undifferentiated-and-differen", "geo_gds_id": "", "name": "Expression data from undifferentiated and differentiated mouse female ES cells PGK12.1", "created": "Nov.12, 2014", "summary": "Affymetrix 430 2.0 mouse arrays were used for expression analyses in undifferentiated and differentiated PGK12.1 ES cells. We found that the X:autosome expression ratios calculated from the mean expression values of X-linked and autosomal genes from microarrays was ~1.4 in undifferentiated female ES cells and then decreased to 1.2 in PGK12.1 cells after 15-day embryoid body differentiation. Thus, a substantial level of X upregulation is already evident in these ES cells prior to differentiation. Our findings based on Affymetrix expression arrays are consistent with microarray analysis from other labs and our RNA-seq analysis of mouse female PGK12.1 ES cells. Mouse female ES cells PGK12.1 were differentiated by the EB (embryoid body) differentiation protocol. The presence of two active X chromosomes in undifferentiated female ES cells and one active X in 15-day differentiated cells was verified by Xist RNA FISH. Total RNA was prepared from undifferentiated and 15-day differentiated PGK12.1 and was used for expression array analyses to examine exprssion of the X chromosome during differentiation.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-44251", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-44251/samples/"}