{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM1079827": {"VARIATION": "Dicer Heterozygote"}}, {"GSM1079827": {"VARIATION": "Dicer Heterozygote"}}, {"GSM1079827": {"VARIATION": "Dicer Heterozygote"}}, {"GSM1079830": {"VARIATION": "Dicer Null"}}, {"GSM1079830": {"VARIATION": "Dicer Null"}}, {"GSM1079830": {"VARIATION": "Dicer Null"}}], "id": 7168, "pop_total": 0, "platform": 6, "summary_wrapped": "MicroRNAs (miRNAs) are critical to proliferation, differentiation, and development. Here, we characterize gene expression in murine...", "geo_gse_id": "E-GEOD-44162", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 6, "tags": ["cell", "fibroblast", "inner cell mass", "sarcoma", "stem cell"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "let-7-represses-nr6a1-and-a-mid-gestation-developm", "geo_id_plat": "E-GEOD-44162_A-AFFY-45", "name": "Let-7 represses Nr6a1 and a mid-gestation developmental program in adult fibroblasts [3p arrays]", "created": "Nov.12, 2014", "summary": "MicroRNAs (miRNAs) are critical to proliferation, differentiation, and development. Here, we characterize gene expression in murine Dicer-null adult mesenchymal stem cell lines, a fibroblast cell type.  Loss of Dicer leads to de-repression of let-7 targets at levels that exceed 10-100 fold with increases in transcription.  Direct and indirect targets of this miRNA belong to a mid-gestation embryonic program that encompasses known oncofetal genes as well as oncogenes not previously associated with an embryonic state.  Surprisingly, this mid-gestation program represents a distinct period that occurs between the pluripotent state of the inner cell mass at embryonic day 3.5 and the induction of let-7, upon differentiation, at embryonic day 10.5.  Within this mid-gestation program, we characterize the let-7 target Nr6a1, an embryonic transcriptional repressor that regulates gene expression in adult fibroblasts following miRNA loss.  In total, let-7 is required for the continual suppression of embryonic gene expression in adult cells, a mechanism that may underlie its tumor suppressive function. Three dicer heterozygotes and 3 dicer null sarcoma cell lines.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-44162", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-44162/samples/"}