{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 7156, "factors": [{"GSM1074697": {"TRANSDUCED WITH": "adeno-EV (empty virus) at x100 MOI for 1h"}}, {"GSM1074698": {"TRANSDUCED WITH": "adeno-Cre at x100 MOI for 1h"}}, {"GSM1074697": {"TRANSDUCED WITH": "adeno-EV (empty virus) at x100 MOI for 1h"}}, {"GSM1074698": {"TRANSDUCED WITH": "adeno-Cre at x100 MOI for 1h"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "PTEN is thought to play a critical role in T cell activation by negatively regulating the PI3K signaling pathway important for cellular...", "pubmed_id": 23851688, "geo_gse_id": "E-GEOD-43936", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 4, "tags": ["cell", "cytokine", "protein", "thymus"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "evaluation-of-effect-of-pten-gene-deletion-in-mous", "geo_id_plat": "E-GEOD-43936_A-AFFY-45", "name": "Evaluation of effect of PTEN gene deletion in mouse CD4+ Th1 clones after stimulation", "created": "Nov.12, 2014", "summary": "PTEN is thought to play a critical role in T cell activation by negatively regulating the PI3K signaling pathway important for cellular activation, growth, and proliferation.  T cells from mice in which PTEN was conditionally deleted in the thymus were reported to display CD28-independent IL-2 production and relative resistance to anergy induction.  However, such observations could have stemmed from alterations in T cell development due to early deletion in thymocytes.  To directly eliminate PTEN in post-thymic T cells, we utilized CAR Tg x PTENflox/flox mice which enabled gene deletion using a Cre adenovirus in vitro.  Gene expression profiling revealed a small subset of induced genes that were augmented upon PTEN deletion and T cell stimulation.  Our results indicate that deletion of PTEN can augment the activation of post-thymic T cells.  Nonetheless, PTEN inhibition may be a viable target for immune potentiation due to increased cytokine production by activated CD4+ cells. It was of interest to determine the spectrum of transcripts regulated by PTEN using gene expression profiling.  To this end, CAR Tg x PTENflox/flox Th1 clones were either treated with adeno-Cre or adeno-EV, then confirmed to have PTEN protein abscence by western blot, then stimulated for 6 hours with anti-CD3/anti-CD28 mAb- coated beads and analyzed (2 total conditions). The experiment was replicated so there are 2 samples for each condition.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-43936", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-43936/samples/"}