{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1067396": {"TREATMENT": "T cells co-cultured with syngeneic dendritic cells"}}, {"GSM1067396": {"TREATMENT": "T cells co-cultured with syngeneic dendritic cells"}}, {"GSM1067396": {"TREATMENT": "T cells co-cultured with syngeneic dendritic cells"}}, {"GSM1067399": {"TREATMENT": "T cells co-cultured with allongeneic dendritic cells"}}, {"GSM1067399": {"TREATMENT": "T cells co-cultured with allongeneic dendritic cells"}}, {"GSM1067399": {"TREATMENT": "T cells co-cultured with allongeneic dendritic cells"}}, {"GSM1067402": {"TREATMENT": "T cells cultivated with anti-CD3e and anti-CD28 mAB9"}}, {"GSM1067402": {"TREATMENT": "T cells cultivated with anti-CD3e and anti-CD28 mAB9"}}, {"GSM1067402": {"TREATMENT": "T cells cultivated with anti-CD3e and anti-CD28 mAB9"}}], "id": 7137, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Molecular networks based on genome-wide mRNA and miRNA expression in activated T cells are interated by co-regulation with miRNA and...", "geo_gse_id": "E-GEOD-43635", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 9, "tags": ["cell", "dendritic", "genome", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-from-mouse-t-cells-after-clip-cros", "geo_id_plat": "E-GEOD-43635_A-AFFY-45", "name": "Expression data from mouse T cells after CLIP (Crosslinking immunoprecipitation) procedures (mRNA)", "created": "Nov.12, 2014", "summary": "Molecular networks based on genome-wide mRNA and miRNA expression in activated T cells are interated by co-regulation with miRNA and protein-coding genes during immune responses, however, accurate prediction of miRs and their targets remains a challenge. Furthermore, the specific changes in the expression of miRNAs and/or mRNAs in allogeneic activated T cells during HCT would be distinct from those responding to non-specific stimulation. Recently, Ago-CLIP has been demonstrated to provide a robust platform for identification of precise miRNA-mRNA interactions by definitively distinguishing direct from indirect miRNA-target interactions. We utilized CLIP procedure followed by screening with standard microarray platforms for miRNA and mRNA transcripts to demonstrate the miR-mRNA landscape and identify novel molecular targets for modulating T cell responses. Mixed cell culture was performed with T cells in the presence of syngeneic 57BL/6, or allogeneic BALB/c dendritic cells or cultivated with anti-CD3e and anti-CD28 mAB9 for 24 h.  Purified T cells were processde for CLIP procedures and followed by RNA isolation (TRIzol LS and miReasy Kit) and hybridization on Affymetrix microarrays and Exiqon 6th generation miRCURY LNA microRNA Arrays . We sought to obtain that the specific changes in the expression of miRNAs and/or mRNAs in allogeneic activated T cells during hematopoietic cells transplantation and that would be distinct from those responding to non-specific stimulation.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-43635", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-43635/samples/"}