{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "human", "factors": [{"GSM1063988": {"SHRNA": "ZEB1", "PHENOTYPE": "epithelial", "CELL LINE": "PC3-EMT1", "CELL TYPE": "derived from PC3-Epi human prostate cancer epithelial cells"}}, {"GSM1063987": {"SHRNA": "scrambled", "PHENOTYPE": "mesenchymal", "CELL LINE": "PC3-EMT1", "CELL TYPE": "derived from PC3-Epi human prostate cancer epithelial cells"}}, {"GSM1063986": {"SHRNA": "ZEB1", "PHENOTYPE": "epithelial", "CELL LINE": "PC3-EMT14", "CELL TYPE": "derived from PC3-Epi human prostate cancer epithelial cells"}}, {"GSM1063985": {"SHRNA": "scrambled", "PHENOTYPE": "mesenchymal", "CELL LINE": "PC3-EMT14", "CELL TYPE": "derived from PC3-Epi human prostate cancer epithelial cells"}}, {"GSM1063984": {"SHRNA": "none", "PHENOTYPE": "mesenchymal", "CELL LINE": "PC3-EMT14", "CELL TYPE": "derived from PC3-Epi human prostate cancer epithelial cells"}}, {"GSM1063983": {"SHRNA": "none", "PHENOTYPE": "mesenchymal", "CELL LINE": "PC3-EMT12", "CELL TYPE": "derived from PC3-Epi human prostate cancer epithelial cells"}}, {"GSM1063982": {"SHRNA": "none", "PHENOTYPE": "mesenchymal", "CELL LINE": "PC3-EMT1", "CELL TYPE": "derived from PC3-Epi human prostate cancer epithelial cells"}}, {"GSM106398": {"SHRNA": "none", "PHENOTYPE": "epithelial", "CELL LINE": "PC3-Epi", "CELL TYPE": "derived from PC3 human prostate cancer epithelial cells"}}], "id": 2398, "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "To study the mechanisms of the epithelial-mesenchymal transition (EMT) in prostate cancer metastasis, we first generated an epithelial...", "geo_gse_id": "E-GEOD-43489", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 4, "sample_count": 8, "tags": ["cancer", "cell", "epithelial phenotype", "line", "mesenchymal cell", "prostate", "prostate cancer"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-profile-from-pce-epi-and-derived-cell-l", "geo_id_plat": "E-GEOD-43489_A-AFFY-44", "name": "Expression profile from PCE-Epi and derived cell lines", "created": "Jul.12, 2014", "summary": "To study the mechanisms of the epithelial-mesenchymal transition (EMT) in prostate cancer metastasis, we first generated an epithelial model cell line derived from prostate cancer epithelial PC3 cells. We isolated a subpopulation of cells that showed a stable epithelial phenotype in culture and designated them PC3-Epi. We next developed mesenchymal cell lines from PC3-Epi cells, through interactions with macrophages. These mesenchymal cell lines were designated PC3-EMT1, PC3-EMT12 and PC3-EMT14. We transfected two of these mesenchymal lines (PC3-EMT1 and PC3-EMT14) with lentiviral ZEB1-shRNA vector (sh4) or the scrambled control (Scr). Consistent with ZEB1's role in EMT and maintenance of the mesenchymal state, silencing of ZEB1 induced the mesenchymal-epithelial transition (MET) in PC3-EMT1 and PC3-EMT14. Cells were plated and allowed to recover for 2 days prior to RNA extraction. PC3-Epi served as a reference sample.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-43489", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-43489/samples/"}