ArrayExpress Uploader0humanZEB1epithelialPC3-EMT1derived from PC3-Epi human prostate cancer epithelial cellsscrambledmesenchymalPC3-EMT1derived from PC3-Epi human prostate cancer epithelial cellsZEB1epithelialPC3-EMT14derived from PC3-Epi human prostate cancer epithelial cellsscrambledmesenchymalPC3-EMT14derived from PC3-Epi human prostate cancer epithelial cellsnonemesenchymalPC3-EMT14derived from PC3-Epi human prostate cancer epithelial cellsnonemesenchymalPC3-EMT12derived from PC3-Epi human prostate cancer epithelial cellsnonemesenchymalPC3-EMT1derived from PC3-Epi human prostate cancer epithelial cellsnoneepithelialPC3-Epiderived from PC3 human prostate cancer epithelial cells2398arrayexpress_sid4To study the mechanisms of the epithelial-mesenchymal transition (EMT) in prostate cancer metastasis, we first generated an epithelial...E-GEOD-43489/profile/8773/arrayexpressuploader48cancercellepithelial phenotypelinemesenchymal cellprostateprostate cancerDec.12, 2014Falseexpression-profile-from-pce-epi-and-derived-cell-lE-GEOD-43489_A-AFFY-44Expression profile from PCE-Epi and derived cell linesJul.12, 2014To study the mechanisms of the epithelial-mesenchymal transition (EMT) in prostate cancer metastasis, we first generated an epithelial model cell line derived from prostate cancer epithelial PC3 cells. We isolated a subpopulation of cells that showed a stable epithelial phenotype in culture and designated them PC3-Epi. We next developed mesenchymal cell lines from PC3-Epi cells, through interactions with macrophages. These mesenchymal cell lines were designated PC3-EMT1, PC3-EMT12 and PC3-EMT14. We transfected two of these mesenchymal lines (PC3-EMT1 and PC3-EMT14) with lentiviral ZEB1-shRNA vector (sh4) or the scrambled control (Scr). Consistent with ZEB1's role in EMT and maintenance of the mesenchymal state, silencing of ZEB1 induced the mesenchymal-epithelial transition (MET) in PC3-EMT1 and PC3-EMT14. Cells were plated and allowed to recover for 2 days prior to RNA extraction. PC3-Epi served as a reference sample.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-43489http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-43489/samples/