ArrayExpress Uploader02244stimulation with aNKp44 and IL-1/IL-7/IL-23stimulation with aNKp44 and IL-1/IL-7/IL-23stimulation with aNKp44 and IL-1/IL-7/IL-23stimulation with aNKp44stimulation with aNKp44stimulation with aNKp44stimulation with IL-1/IL-7/IL-23stimulation with IL-1/IL-7/IL-23stimulation with IL-1/IL-7/IL-23ex-vivoex-vivoarrayexpress_sid4RORγt+ innate lymphoid cells (ILC) are crucial players of innate immune responses and represent a major source of IL-22, which has an...23791642E-GEOD-43409/profile/8773/arrayexpressuploader111cytokinetonsilDec.12, 2014Falserort-innate-lymphoid-cells-transcriptomes-after-anE-GEOD-43409_A-AFFY-44RORγt+ Innate lymphoid cells transcriptomes after aNKp44 and cytokine stimulationJul.11, 2014RORγt+ innate lymphoid cells (ILC) are crucial players of innate immune responses and represent a major source of IL-22, which has an important role in mucosal homeostasis. The signals required by RORγt+ ILC to express IL-22 and other cytokines, including TNF, have only partially been elucidated. Here we show that RORγt+ ILC can directly sense the environment by the engagement of the activating receptor NKp44. NKp44 triggering in RORγt+ ILC selectively activates a coordinated pro-inflammatory program, including TNF, while cytokine stimulation induces preferentially IL-22 expression. However, combined engagement of NKp44 and cytokine receptors results in a strong synergistic effect. These data support the concept that NKp44+ RORγt+ ILC can be activated without cytokines and are able to switch between IL-22 or TNF production, depending on the triggering stimulus. Transcriptome analysis of CD56+CD127hi tonsil ILC, Ex vivo and after stimulation with aNKp44, IL-1/IL-7/IL-23 or aNKp44/IL-1/IL-7/IL-23 for 3.5h. RNA was extracted and pooled from 2 donors each, Amplified and labeled according to manufacturer´s instructions (GeneChipU133plus2® , Affymetrix). The analysis was performed in triplicates.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-43409humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-43409/samples/