{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 2244, "factors": [{"GSM1062207": {"TREATMENT PROTOCOL": "stimulation with aNKp44 and IL-1/IL-7/IL-23"}}, {"GSM1062207": {"TREATMENT PROTOCOL": "stimulation with aNKp44 and IL-1/IL-7/IL-23"}}, {"GSM1062207": {"TREATMENT PROTOCOL": "stimulation with aNKp44 and IL-1/IL-7/IL-23"}}, {"GSM1062204": {"TREATMENT PROTOCOL": "stimulation with aNKp44"}}, {"GSM1062204": {"TREATMENT PROTOCOL": "stimulation with aNKp44"}}, {"GSM1062204": {"TREATMENT PROTOCOL": "stimulation with aNKp44"}}, {"GSM106220": {"TREATMENT PROTOCOL": "stimulation with IL-1/IL-7/IL-23"}}, {"GSM106220": {"TREATMENT PROTOCOL": "stimulation with IL-1/IL-7/IL-23"}}, {"GSM106220": {"TREATMENT PROTOCOL": "stimulation with IL-1/IL-7/IL-23"}}, {"GSM1062198": {"TREATMENT PROTOCOL": "ex-vivo"}}, {"GSM1062198": {"TREATMENT PROTOCOL": "ex-vivo"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 4, "summary_wrapped": "ROR\u03b3t+ innate lymphoid cells (ILC) are crucial players of innate immune responses and represent a major source of IL-22, which has an...", "pubmed_id": 23791642, "geo_gse_id": "E-GEOD-43409", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 11, "tags": ["cytokine", "tonsil"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "rort-innate-lymphoid-cells-transcriptomes-after-an", "geo_id_plat": "E-GEOD-43409_A-AFFY-44", "name": "ROR\u03b3t+ Innate lymphoid cells transcriptomes after aNKp44 and cytokine stimulation", "created": "Jul.11, 2014", "summary": "ROR\u03b3t+ innate lymphoid cells (ILC) are crucial players of innate immune responses and represent a major source of IL-22, which has an important role in mucosal homeostasis. The signals required by ROR\u03b3t+ ILC to express IL-22 and other cytokines, including TNF, have only partially been elucidated. Here we show that ROR\u03b3t+ ILC can directly sense the environment by the engagement of the activating receptor NKp44. NKp44 triggering in ROR\u03b3t+ ILC selectively activates a coordinated pro-inflammatory program, including TNF, while cytokine stimulation induces preferentially IL-22 expression. However, combined engagement of NKp44 and cytokine receptors results in a strong synergistic effect. These data support the concept that NKp44+ ROR\u03b3t+ ILC can be activated without cytokines and are able to switch between IL-22 or TNF production, depending on the triggering stimulus. Transcriptome analysis of CD56+CD127hi tonsil ILC, Ex vivo and after stimulation with aNKp44, IL-1/IL-7/IL-23 or aNKp44/IL-1/IL-7/IL-23 for 3.5h. RNA was extracted and pooled from 2 donors each, Amplified and labeled according to manufacturer\u00b4s instructions (GeneChipU133plus2\u00ae , Affymetrix). The analysis was performed in triplicates.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-43409", "species": "human", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-43409/samples/"}