4ArrayExpress Uploader02479arrayexpress_sidhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-43068The MLL gene on chromosome 11 fuses to the AF6 gene on chromosome 6 in a balanced chromosomal translocation that is characetristic of...23361907/profile/8773/arrayexpressuploader01bonebone marrowcellchromosomeleukemialineDec.12, 2014Falseexpression-data-from-a-human-mll-af6-positive-amlE-GEOD-43068_A-AFFY-44Expression data from a human MLL-AF6 positive AML cell lineJul.12, 2014The MLL gene on chromosome 11 fuses to the AF6 gene on chromosome 6 in a balanced chromosomal translocation that is characetristic of certain adult and pediatric human leukemias. We established a murine leukemia model of MLL-AF6 using the retroviral MLL-AF6 contruct in a bone marrow transplantation system. The ML2 human MLL-AF6 positive leukemia cell line was used for gene expression profiling to assess the transctiptional profile in MLL-AF6 leukemias. RNA was extracted from the ML2 cell line cultured under standard conditionswith TRIZOL (Invitrogen), purified using RNAeasy(Qiagen) and hybridized onto Affymetrix arrays.E-GEOD-43068humanhttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-43068/samples/