{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1052653": {"TREATMENT": "sham", "TIME": "0hr"}}, {"GSM1052653": {"TREATMENT": "sham", "TIME": "0hr"}}, {"GSM1052653": {"TREATMENT": "sham", "TIME": "0hr"}}, {"GSM1052656": {"TREATMENT": "flow", "TIME": "0hr"}}, {"GSM1052656": {"TREATMENT": "flow", "TIME": "0hr"}}, {"GSM1052656": {"TREATMENT": "flow", "TIME": "0hr"}}, {"GSM1052659": {"TREATMENT": "sham", "TIME": "2hr"}}, {"GSM1052659": {"TREATMENT": "sham", "TIME": "2hr"}}, {"GSM1052659": {"TREATMENT": "sham", "TIME": "2hr"}}, {"GSM1052662": {"TREATMENT": "flow", "TIME": "2hr"}}, {"GSM1052662": {"TREATMENT": "flow", "TIME": "2hr"}}, {"GSM1052662": {"TREATMENT": "flow", "TIME": "2hr"}}, {"GSM1052665": {"TREATMENT": "sham", "TIME": "8hr"}}, {"GSM1052665": {"TREATMENT": "sham", "TIME": "8hr"}}, {"GSM1052665": {"TREATMENT": "sham", "TIME": "8hr"}}, {"GSM1052668": {"TREATMENT": "flow", "TIME": "8hr"}}, {"GSM1052668": {"TREATMENT": "flow", "TIME": "8hr"}}, {"GSM1052668": {"TREATMENT": "flow", "TIME": "8hr"}}, {"GSM105267": {"TREATMENT": "sham", "TIME": "24hr"}}, {"GSM105267": {"TREATMENT": "sham", "TIME": "24hr"}}, {"GSM105267": {"TREATMENT": "sham", "TIME": "24hr"}}, {"GSM1052674": {"TREATMENT": "flow", "TIME": "24hr"}}, {"GSM1052674": {"TREATMENT": "flow", "TIME": "24hr"}}, {"GSM1052674": {"TREATMENT": "flow", "TIME": "24hr"}}], "id": 8677, "ownerprofile_id": "arrayexpress_sid", "platform": 8, "summary_wrapped": "Osteocytes, positioned within bone\u2019s interstitial space, are subject to fluid flow upon whole bone loading.  Such fluid flow is widely...", "geo_gse_id": "E-GEOD-42874", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 24, "tags": ["bone", "cell", "interstitial space", "osteocyte", "point", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-42874_A-AFFY-36", "slug": "integrative-transcriptomic-and-proteomic-analysis", "geo_gds_id": "", "name": "Integrative transcriptomic and proteomic analysis of osteocytic cells exposed to fluid flow reveals novel mechano-sensitive signaling pathways", "created": "Nov.24, 2014", "summary": "Osteocytes, positioned within bone\u2019s interstitial space, are subject to fluid flow upon whole bone loading.  Such fluid flow is widely theorized to be a mechanical signal transduced by osteocytes, initiating a poorly understood cascade of signaling events mediating bone metabolism.  The objective of this study was to utilize high-throughput approaches to examine the time course of flow-induced changes in osteocyte gene transcript and protein levels. Microarray analysis demonstrated fluid flow regulation of genes consistent with known anabolic loading responses, including Ptgs2, NF-\u03baB inhibitors, MAP3 kinases, and Wnt/\u03b2-catenin pathway signaling molecules.  However, two of the most highly up-regulated gene products\u2014Cxcl1 and Cxcl2, confirmed by qPCR\u2014have not previously been reported to be responsive to fluid flow.  Gene ontology analysis suggested a highly significant inflammatory and immune response, with cellular functions including trafficking, cell-to-cell signaling, and tissue development.  Proteomic analysis of the same samples demonstrated greatest up-regulation of the ATP-producing enzyme NDK, calcium-binding Calcyclin, and G protein-coupled receptor kinase 6.  An integrative pathway analysis merging fold changes in transcript and protein levels predicted signaling nodes not directly detected at the sampled time points, including STAT3 and c-Myc.  These results extend our knowledge of the osteocytic response to fluid flow, most notably up-regulation of Cxcl1 and Cxcl2 as a possible paracrine agent for osteoblastic and osteoclastic recruitment. Osteocyte-like MLO-Y4 cells were subjected to 2 hours of 10 dyn/cm2 oscillating fluid flow in parallel-plate fluid flow chambers and harvested for analysis at 0, 2, 8, and 24 hours post-flow incubation.  Parallel control samples from sham treated cells were also collected at each time point.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42874", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42874/samples/"}