{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 7102, "factors": [{"GSM1051138": {"TREATMENT": "no activation", "STRAIN OR LINE": "C57BL6N"}}, {"GSM1051138": {"TREATMENT": "no activation", "STRAIN OR LINE": "C57BL6N"}}, {"GSM1051138": {"TREATMENT": "no activation", "STRAIN OR LINE": "C57BL6N"}}, {"GSM105114": {"TREATMENT": "no activation", "STRAIN OR LINE": "pLck-hCD1dTg"}}, {"GSM105114": {"TREATMENT": "no activation", "STRAIN OR LINE": "pLck-hCD1dTg"}}, {"GSM105114": {"TREATMENT": "no activation", "STRAIN OR LINE": "pLck-hCD1dTg"}}, {"GSM1051144": {"TREATMENT": "activated in vitro for 6 hours", "STRAIN OR LINE": "C57BL6N"}}, {"GSM1051144": {"TREATMENT": "activated in vitro for 6 hours", "STRAIN OR LINE": "C57BL6N"}}, {"GSM1051144": {"TREATMENT": "activated in vitro for 6 hours", "STRAIN OR LINE": "C57BL6N"}}, {"GSM1051147": {"TREATMENT": "activated in vitro for 6 hours", "STRAIN OR LINE": "pLck-hCD1dTg"}}, {"GSM1051147": {"TREATMENT": "activated in vitro for 6 hours", "STRAIN OR LINE": "pLck-hCD1dTg"}}, {"GSM1051147": {"TREATMENT": "activated in vitro for 6 hours", "STRAIN OR LINE": "pLck-hCD1dTg"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "CD1d expression by thymocytes is required to select iNKT cells. When CD1d is expressed only on thymocytes (pLck-CD1d tg mice), iNKT cells...", "pubmed_id": 23427253, "geo_gse_id": "E-GEOD-42835", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 12, "tags": ["cell", "peripheral", "thymus", "tyrosine"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "expression-data-of-hepatic-inkt-cells-from-plck-hc", "geo_id_plat": "E-GEOD-42835_A-AFFY-45", "name": "Expression data of hepatic iNKT cells from pLck-hCD1d transgenic mice", "created": "Nov.12, 2014", "summary": "CD1d expression by thymocytes is required to select iNKT cells. When CD1d is expressed only on thymocytes (pLck-CD1d tg mice), iNKT cells are hyperresponsive to antigen stimulation suggesting that, in physiological conditions, these cells undergo functional education mediated by additional CD1d-expressing cells. Here, we investigated the mechanisms of this functional education. We find that peripheral iNKT cells from pLck-CD1d tg mice express significantly less SHP-1, a tyrosine phosphatase negatively regulating TCR signaling, than WT cells. iNKT cells from heterozygous SHP-1-mutated motheaten mice, displaying similar SHP-1 reduction as pLck-CD1d tg iNKT cells, are antigen-hyperresponsive. Restoring normal CD1d expression in pLck-CD1d tg mice normalizes SHP-1 expression and responsiveness of iNKT cells. In WT mice, iNKT cells upregulate SHP-1 and decrease responsiveness upon emigration from thymus to periphery. This depends on contacts with CD1d-expressing DCs. iNKT cell functional education is therefore controlled by DCs via tuning SHP-1 expression level in the periphery. Hepatic iNKT cells from wild-type and transgenic mice (expressing hCD1d molecule under the pLck promoter)", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42835", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42835/samples/"}