ArrayExpress Uploaderarrayexpress_sidmouseRKIP-transgenic (expressing RKIP under control of the myocardium-specific alpha-MHC promoter)RKIP-transgenic (expressing RKIP under control of the myocardium-specific alpha-MHC promoter)GRK-Inh-transgenic (myocardium-specific expression of a GRK-specific peptide inhibitor)GRK-Inh-transgenic (myocardium-specific expression of a GRK-specific peptide inhibitor)wild type (control)wild type (control)709706The Raf kinase inhibitor protein (RKIP) is a dual inhibitor of the Raf kinase and the G-protein-coupled receptor kinase 2 (GRK2). GRK2 is...E-GEOD-42753/profile/8773/arrayexpressuploader16heartmyocardiumproteinDec.12, 2014Falsemicroarray-gene-expression-profiling-of-transgenicE-GEOD-42753_A-AFFY-45Microarray gene expression profiling of transgenic mice with myocardium-specific expression of RKIP or a GRK-specific peptide inhibitorNov.12, 2014The Raf kinase inhibitor protein (RKIP) is a dual inhibitor of the Raf kinase and the G-protein-coupled receptor kinase 2 (GRK2). GRK2 is an indispensable kinase, which exerts a major role in the pathogenesis of heart failure, and inhibition of GRK2 is cardioprotective in experimental models of heart failure. To investigate the cardiac function of RKIP as GRK2 inhibitor, we generated transgenic mice with myocardium-specific expression of RKIP under control of the alpha-MHC promoter. For comparison, mice with myocardium-specific expression of a GRK-specific peptide inhibitor (GRK-Inh) were also generated. Two different transgenic mouse models were established. Transgenic RKIP mice and transgenic GRK-Inh mice were born at Mendelian frequencey and grew to adulthood normally. Microarray gene expression profiling was performed with heart tissue isolated from three study groups: (i) RKIP-transgenic mice, (ii) GRK-Inh-transgenic mice, and (iii) B6 control mice.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42753http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42753/samples/