ArrayExpress Uploader070892iESC-like cellsPrdm14 Knockout, pPyCAG-AGP14 transgene2iESC-like cellsPrdm14 Knockout, pPyCAG-AGP14 transgene2iESC-like cellsPrdm14 Knockout2iESC-like cellsPrdm14 Knockout2iESCsNo genetic modification2iESCsNo genetic modificationnot specifiedepiblast like cells (EpiLCs)Blimp1-mVenus, Stella-ECFP(BVSC) transgenenot specifiedepiblast like cells (EpiLCs)Blimp1-mVenus, Stella-ECFP(BVSC) transgenenot specifiedepiblast like cells (EpiLCs)Blimp1-mVenus, Stella-ECFP(BVSC) transgenenot specifiedepiblast like cells (EpiLCs)Blimp1-mVenus, Stella-ECFP(BVSC) transgeneSerum without LIF (day2)ESC-like cellsPrdm14 Knockout, pPyCAG-AGP14 transgeneSerum without LIF (day2)ESC-like cellsPrdm14 Knockout, pPyCAG-AGP14 transgeneSerum without LIF (day2)ESC-like cellsPrdm14 KnockoutSerum without LIF (day2)ESC-like cellsPrdm14 KnockoutSerum without LIF (day2)ESCsNo genetic modificationSerum without LIF (day2)ESCsNo genetic modificationSerum (day2)ESC-like cellsPrdm14 Knockout, pPyCAG-AGP14 transgeneSerum (day2)ESC-like cellsPrdm14 Knockout, pPyCAG-AGP14 transgeneSerum (day2)ESC-like cellsPrdm14 KnockoutSerum (day2)ESC-like cellsPrdm14 KnockoutSerum (day2)ESCsNo genetic modificationSerum (day2)ESCsNo genetic modificationSerum+MEK inhibitor (day2)ESC-like cellsPrdm14 KnockoutSerum+MEK inhibitor (day2)ESC-like cellsPrdm14 KnockoutSerum+MEK inhibitor (day2)ESCsNo genetic modificationSerum+MEK inhibitor (day2)ESCsNo genetic modificationarrayexpress_sid6Mouse embryonic stem cells (mESCs) fluctuate between a naïve inner cell mass (ICM)-like state and a primed epiblast-like state of...23333148E-GEOD-42580/profile/8773/arrayexpressuploader326cellfibroblastglycogeninner cell massproteinserumDec.12, 2014Falseprdm14-ensures-a-naive-pluripotency-by-a-dual-mechE-GEOD-42580_A-AFFY-45PRDM14 Ensures a Naïve Pluripotency by a Dual Mechanism Involving Signaling and Epigenetic Pathways in Mouse Embryonic Stem CellsNov.12, 2014Mouse embryonic stem cells (mESCs) fluctuate between a naïve inner cell mass (ICM)-like state and a primed epiblast-like state of pluripotency in serum, but are harnessed exclusively in a distinctive, apparently more naïve state of pluripotency (the ground state) with inhibitors for mitogen-activated protein kinase (MAPK) and glycogen synthase kinase 3 pathways (2i). Understanding the mechanism ensuring a naïve state of pluripotency would be critical in realizing a full potential of ESCs. We show here that PRDM14, a PR domain-containing transcriptional regulator, ensures a naïve pluripotency by a dual mechanism: Antagonizing fibroblast growth factor receptor (FGFR) signaling that is activated paradoxically by the core transcriptional circuitry for pluripotency and directs a primed state and repressing de novo DNA methyltransferases that create a primed epiblast-like epigenome. PRDM14 exerts these functions by recruiting polycomb repressive complex 2 (PRC2) specifically to key targets and repressing their expression. Mouse Embryonic Stem Cells (mESCs) or mESC-like cells with different Prdm14 genotypes {Prdm14(+/+), Prdm14(-/-), and Prdm14(-/-) rescued with Avitag-EGFP-Prdm14 transgene [Prdm14(-/-)+AGP14]} are cultured on MEF in different medium [2i, Serum(day 2), Serum+MEK inhibitor (PD0325901) (day 2), Serum without LIF (day2)].http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42580mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42580/samples/