{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 7089, "factors": [{"GSM1045422": {"CULTURE CONDITIONS": "2i", "CELL TYPE": "ESC-like cells", "VARIATION": "Prdm14 Knockout, pPyCAG-AGP14 transgene"}}, {"GSM1045422": {"CULTURE CONDITIONS": "2i", "CELL TYPE": "ESC-like cells", "VARIATION": "Prdm14 Knockout, pPyCAG-AGP14 transgene"}}, {"GSM1045424": {"CULTURE CONDITIONS": "2i", "CELL TYPE": "ESC-like cells", "VARIATION": "Prdm14 Knockout"}}, {"GSM1045424": {"CULTURE CONDITIONS": "2i", "CELL TYPE": "ESC-like cells", "VARIATION": "Prdm14 Knockout"}}, {"GSM1045426": {"CULTURE CONDITIONS": "2i", "CELL TYPE": "ESCs", "VARIATION": "No genetic modification"}}, {"GSM1045426": {"CULTURE CONDITIONS": "2i", "CELL TYPE": "ESCs", "VARIATION": "No genetic modification"}}, {"GSM1045428": {"CULTURE CONDITIONS": "not specified", "CELL TYPE": "epiblast like cells (EpiLCs)", "VARIATION": "Blimp1-mVenus, Stella-ECFP(BVSC) transgene"}}, {"GSM1045428": {"CULTURE CONDITIONS": "not specified", "CELL TYPE": "epiblast like cells (EpiLCs)", "VARIATION": "Blimp1-mVenus, Stella-ECFP(BVSC) transgene"}}, {"GSM1045428": {"CULTURE CONDITIONS": "not specified", "CELL TYPE": "epiblast like cells (EpiLCs)", "VARIATION": "Blimp1-mVenus, Stella-ECFP(BVSC) transgene"}}, {"GSM1045428": {"CULTURE CONDITIONS": "not specified", "CELL TYPE": "epiblast like cells (EpiLCs)", "VARIATION": "Blimp1-mVenus, Stella-ECFP(BVSC) transgene"}}, {"GSM1045432": {"CULTURE CONDITIONS": "Serum without LIF (day2)", "CELL TYPE": "ESC-like cells", "VARIATION": "Prdm14 Knockout, pPyCAG-AGP14 transgene"}}, {"GSM1045432": {"CULTURE CONDITIONS": "Serum without LIF (day2)", "CELL TYPE": "ESC-like cells", "VARIATION": "Prdm14 Knockout, pPyCAG-AGP14 transgene"}}, {"GSM1045434": {"CULTURE CONDITIONS": "Serum without LIF (day2)", "CELL TYPE": "ESC-like cells", "VARIATION": "Prdm14 Knockout"}}, {"GSM1045434": {"CULTURE CONDITIONS": "Serum without LIF (day2)", "CELL TYPE": "ESC-like cells", "VARIATION": "Prdm14 Knockout"}}, {"GSM1045436": {"CULTURE CONDITIONS": "Serum without LIF (day2)", "CELL TYPE": "ESCs", "VARIATION": "No genetic modification"}}, {"GSM1045436": {"CULTURE CONDITIONS": "Serum without LIF (day2)", "CELL TYPE": "ESCs", "VARIATION": "No genetic modification"}}, {"GSM1045438": {"CULTURE CONDITIONS": "Serum (day2)", "CELL TYPE": "ESC-like cells", "VARIATION": "Prdm14 Knockout, pPyCAG-AGP14 transgene"}}, {"GSM1045438": {"CULTURE CONDITIONS": "Serum (day2)", "CELL TYPE": "ESC-like cells", "VARIATION": "Prdm14 Knockout, pPyCAG-AGP14 transgene"}}, {"GSM1045440": {"CULTURE CONDITIONS": "Serum (day2)", "CELL TYPE": "ESC-like cells", "VARIATION": "Prdm14 Knockout"}}, {"GSM1045440": {"CULTURE CONDITIONS": "Serum (day2)", "CELL TYPE": "ESC-like cells", "VARIATION": "Prdm14 Knockout"}}, {"GSM1045442": {"CULTURE CONDITIONS": "Serum (day2)", "CELL TYPE": "ESCs", "VARIATION": "No genetic modification"}}, {"GSM1045442": {"CULTURE CONDITIONS": "Serum (day2)", "CELL TYPE": "ESCs", "VARIATION": "No genetic modification"}}, {"GSM1045444": {"CULTURE CONDITIONS": "Serum+MEK inhibitor (day2)", "CELL TYPE": "ESC-like cells", "VARIATION": "Prdm14 Knockout"}}, {"GSM1045444": {"CULTURE CONDITIONS": "Serum+MEK inhibitor (day2)", "CELL TYPE": "ESC-like cells", "VARIATION": "Prdm14 Knockout"}}, {"GSM1045446": {"CULTURE CONDITIONS": "Serum+MEK inhibitor (day2)", "CELL TYPE": "ESCs", "VARIATION": "No genetic modification"}}, {"GSM1045446": {"CULTURE CONDITIONS": "Serum+MEK inhibitor (day2)", "CELL TYPE": "ESCs", "VARIATION": "No genetic modification"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "Mouse embryonic stem cells (mESCs) fluctuate between a na\u00efve inner cell mass (ICM)-like state and a primed epiblast-like state of...", "pubmed_id": 23333148, "geo_gse_id": "E-GEOD-42580", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 26, "tags": ["cell", "fibroblast", "glycogen", "inner cell mass", "protein", "serum"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "prdm14-ensures-a-naive-pluripotency-by-a-dual-mech", "geo_id_plat": "E-GEOD-42580_A-AFFY-45", "name": "PRDM14 Ensures a Na\u00efve Pluripotency by a Dual Mechanism Involving Signaling and Epigenetic Pathways in Mouse Embryonic Stem Cells", "created": "Nov.12, 2014", "summary": "Mouse embryonic stem cells (mESCs) fluctuate between a na\u00efve inner cell mass (ICM)-like state and a primed epiblast-like state of pluripotency in serum, but are harnessed exclusively in a distinctive, apparently more na\u00efve state of pluripotency (the ground state) with inhibitors for mitogen-activated protein kinase (MAPK) and glycogen synthase kinase 3 pathways (2i).  Understanding the mechanism ensuring a na\u00efve state of pluripotency would be critical in realizing a full potential of ESCs.  We show here that PRDM14, a PR domain-containing transcriptional regulator, ensures a na\u00efve pluripotency by a dual mechanism: Antagonizing fibroblast growth factor receptor (FGFR) signaling that is activated paradoxically by the core transcriptional circuitry for pluripotency and directs a primed state and repressing de novo DNA methyltransferases that create a primed epiblast-like epigenome.  PRDM14 exerts these functions by recruiting polycomb repressive complex 2 (PRC2) specifically to key targets and repressing their expression. Mouse Embryonic Stem Cells (mESCs) or mESC-like cells with different Prdm14 genotypes {Prdm14(+/+), Prdm14(-/-), and Prdm14(-/-) rescued with Avitag-EGFP-Prdm14 transgene [Prdm14(-/-)+AGP14]} are cultured on MEF in different medium [2i, Serum(day 2), Serum+MEK inhibitor (PD0325901) (day 2), Serum without LIF (day2)].", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42580", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42580/samples/"}