{"owner": "ArrayExpress Uploader", "pop_total": 0, "id": 7085, "factors": [{"GSM1040577": {"SHRNA": "Vector control"}}, {"GSM1040578": {"SHRNA": "shPhf19"}}, {"GSM1040577": {"SHRNA": "Vector control"}}, {"GSM1040578": {"SHRNA": "shPhf19"}}], "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "PCL family protein Phf19/Pcl3 is one of the accessory components of the PRC2 core complex, and Phf19 is highly expressed in murine ES...", "pubmed_id": 23273982, "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 4, "tags": ["carcinoma", "cell", "embryonal carcinoma", "line", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_id_plat": "E-GEOD-42463_A-AFFY-45", "slug": "phf19-knockdown-effect-on-f9-embryonal-carcinoma-c", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42463", "geo_gds_id": "", "name": "Phf19 knockdown effect on F9 embryonal carcinoma cells", "created": "Nov.12, 2014", "summary": "PCL family protein Phf19/Pcl3 is one of the accessory components of the PRC2 core complex, and Phf19 is highly expressed in murine ES cells and an ES cell-like embryonic carcinoma cell line, F9 cells. Here we performed microarray analysis of embryonal carcinoma cell line F9 following Phf19 knockdown by shRNA. Knocking down Phf19/Pcl3 in F9 embryonic cells led to derepression of numerous PRC2 direct target genes. 4 sampels including 2 shRNA vector control cell lines and 2 shPhf19 cell lines were used for RNA extraction and Affymetrix mouse 430 2.0 arrays.", "geo_gse_id": "E-GEOD-42463", "species": "mouse", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42463/samples/"}