{"owner": "ArrayExpress Uploader", "ownerprofile_id": "arrayexpress_sid", "species": "mouse", "factors": [{"GSM1037447": {"TREATMENT": "unilateral ureteral obstruction", "GENOTYPE": "WT"}}, {"GSM1037447": {"TREATMENT": "unilateral ureteral obstruction", "GENOTYPE": "WT"}}, {"GSM1037447": {"TREATMENT": "unilateral ureteral obstruction", "GENOTYPE": "WT"}}, {"GSM1037450": {"TREATMENT": "unilateral ureteral obstruction", "GENOTYPE": "TIMP2 -/-"}}, {"GSM1037450": {"TREATMENT": "unilateral ureteral obstruction", "GENOTYPE": "TIMP2 -/-"}}, {"GSM1037450": {"TREATMENT": "unilateral ureteral obstruction", "GENOTYPE": "TIMP2 -/-"}}, {"GSM1037453": {"TREATMENT": "unilateral ureteral obstruction", "GENOTYPE": "TIMP3 -/-"}}, {"GSM1037453": {"TREATMENT": "unilateral ureteral obstruction", "GENOTYPE": "TIMP3 -/-"}}, {"GSM1037453": {"TREATMENT": "unilateral ureteral obstruction", "GENOTYPE": "TIMP3 -/-"}}, {"GSM1037456": {"TREATMENT": "sham", "GENOTYPE": "WT"}}, {"GSM1037456": {"TREATMENT": "sham", "GENOTYPE": "WT"}}, {"GSM1037458": {"TREATMENT": "sham", "GENOTYPE": "TIMP2 -/-"}}, {"GSM1037458": {"TREATMENT": "sham", "GENOTYPE": "TIMP2 -/-"}}, {"GSM1037458": {"TREATMENT": "sham", "GENOTYPE": "TIMP2 -/-"}}, {"GSM103746": {"TREATMENT": "sham", "GENOTYPE": "TIMP3 -/-"}}, {"GSM103746": {"TREATMENT": "sham", "GENOTYPE": "TIMP3 -/-"}}, {"GSM103746": {"TREATMENT": "sham", "GENOTYPE": "TIMP3 -/-"}}, {"GSM1037456": {"TREATMENT": "sham", "GENOTYPE": "WT"}}], "id": 8666, "pop_total": 0, "platform": 8, "summary_wrapped": "Tissue inhibitors of metalloproteinases (TIMP) are endogenous inhibitors of matrix metalloproteinases (MMP). While TIMP2 and TIMP3...", "pubmed_id": 23760282, "geo_gse_id": "E-GEOD-42303", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 18, "tags": ["collagen", "intermediate", "kidney", "ureteral obstruction"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "timp2-and-timp3-have-divergent-roles-in-early-rena", "geo_id_plat": "E-GEOD-42303_A-AFFY-36", "name": "TIMP2 and TIMP3 have divergent roles in early renal tubulointerstitial injury", "created": "Nov.24, 2014", "summary": "Tissue inhibitors of metalloproteinases (TIMP) are endogenous inhibitors of matrix metalloproteinases (MMP). While TIMP2 and TIMP3 inhibit MMPs, TIMP3 also inhibits activation of pro-MMP2 whereas TIMP2 promotes it. Here we assessed the differential role of TIMP2 and TIMP3 in renal injury using the unilateral ureteral obstruction model. Gene microarray assay showed that post-obstruction, the lack of TIMP3 had a greater impact on gene expression of intermediate, late injury- and repair-induced transcripts, kidney selective transcripts and solute carriers. Renal injury in TIMP3-/-, but not in TIMP2-/- mice increased expression of collagen type I/III, connective tissue growth factor, transforming growth factor-\u03b2 and the downstream Smad2/3 pathway. Interestingly, ureteral obstruction markedly increased MMP2 activation in the kidneys of  TIMP3-/- mice which was completely blocked in the kidneys of TIMP2-/- mice. These changes are consistent with enhanced renal tubulointerstitial fibrosis in TIMP3-/- and its reduction in TIMP2-/- mice. The activity of tumor necrosis factor-\u03b1 converting enzyme, caspase-3 and mitogen activated kinases were elevated in the kidneys of  TIMP3-/- but not TIMP2-/- mice, suggesting enhanced activation of apoptotic and pathological signaling pathways only in the obstructed kidney of  TIMP3-/- mice. Thus, TIMP2 and TIMP3 play differential and contrasting roles in renal injury, TIMP3 protects from damage whereas TIMP2 promotes injury through MMP2 activation. Kidneys from the wild type (WT), TIMP2-/- and TIMP3-/- mice undergoing sham or unilateral ureteral obstruction (UUO) procedures", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42303", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42303/samples/"}