{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1036395": {"GENOTYPE": "MLL-AF9 EF1\u03b1wt/wt ROSAwt/CreERT2", "SEX": "male"}}, {"GSM1036395": {"GENOTYPE": "MLL-AF9 EF1\u03b1wt/wt ROSAwt/CreERT2", "SEX": "male"}}, {"GSM1036395": {"GENOTYPE": "MLL-AF9 EF1\u03b1wt/wt ROSAwt/CreERT2", "SEX": "male"}}, {"GSM1036398": {"GENOTYPE": "MLL-AF9 EF1\u03b1wt/lsl-N1-IC ROSAwt/CreERT2", "SEX": "female"}}, {"GSM1036398": {"GENOTYPE": "MLL-AF9 EF1\u03b1wt/lsl-N1-IC ROSAwt/CreERT2", "SEX": "female"}}, {"GSM1036398": {"GENOTYPE": "MLL-AF9 EF1\u03b1wt/lsl-N1-IC ROSAwt/CreERT2", "SEX": "female"}}], "id": 7080, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "To determine role of Notch signaling in AML leukemia initiating cells we used a conditional mouse knock-in model of Notch1-IC to induce...", "geo_gse_id": "E-GEOD-42260", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 2, "sample_count": 6, "tags": ["body", "bone", "bone marrow", "leukemia", "notch"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "notch-pathway-activation-targets-aml-cell-homeos-2", "geo_id_plat": "E-GEOD-42260_A-AFFY-45", "name": "Notch Pathway Activation Targets AML Cell Homeostasis and Differentiation: MLL-AF9 transformed LGMP", "created": "Nov.12, 2014", "summary": "To determine role of Notch signaling in AML leukemia initiating cells we used a conditional mouse knock-in model of Notch1-IC to induce Notch1-IC expression in MLL-AF9 transformed LGMP. WT and Notch1-IC+ LGMP were analyzed to determined genes controlled by Notch signaling. 12 weeks old wt lethaly irradiated mice were transplanted with 50000 cKit+ MLL-AF9-IRES-YFP infected cells from MLL-AF9 EF1 wt/wt ROSAwt/CreERT2 or MLL-AF9 EF1 wt/lsl-N1-IC ROSAwt/CreERT2 mice + 250000 support wt total bone marrow cells. 4 weeks after transplant mice were injected 2 times with tamoxifen (0.2mg/g body weight) every other day. Mice were sacrificed and analyzed 6 days after last injection. LGMP were flow purified for RNA extraction and hybridization on Affymetrix microarrays.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42260", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42260/samples/"}