{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM104087": {"VARIATION": "Cbfb\u0394C95/\u0394C95"}}, {"GSM104087": {"VARIATION": "Cbfb\u0394C95/\u0394C95"}}, {"GSM104087": {"VARIATION": "Cbfb\u0394C95/\u0394C95"}}, {"GSM1040874": {"VARIATION": "Cbfb+/\u0394C95"}}, {"GSM1040874": {"VARIATION": "Cbfb+/\u0394C95"}}, {"GSM1040874": {"VARIATION": "Cbfb+/\u0394C95"}}, {"GSM1040877": {"VARIATION": "Cbfb+/+"}}, {"GSM1040877": {"VARIATION": "Cbfb+/+"}}, {"GSM1040877": {"VARIATION": "Cbfb+/+"}}], "id": 7079, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "The C-terminus of CBF\u00df-SMMHC, the fusion protein produced by a chromosome 16 inversion in acute myeloid leukemia subtype M4Eo, contains...", "geo_gse_id": "E-GEOD-42238", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 1, "sample_count": 9, "tags": ["acute myeloid leukemia", "chromosome", "leukemia", "myeloid leukemia", "protein"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "the-c-terminus-of-cbf-smmhc-is-required-to-induce", "geo_id_plat": "E-GEOD-42238_A-AFFY-45", "name": "The C-terminus of CBF\u00df-SMMHC is required to induce embryonic hematopoietic defects and leukemogenesis.", "created": "Nov.12, 2014", "summary": "The C-terminus of CBF\u00df-SMMHC, the fusion protein produced by a chromosome 16 inversion in acute myeloid leukemia subtype M4Eo, contains domains for self-mulimerization and transcriptional repression, both of which have been proposed to be important for leukemogenesis by CBF\u00df-SMMHC. To test the role of the fusion protein's C-terminus in vivo, we generated knock-in mice expressing a C-terminally truncated CBF\u00df-SMMHC (CBF\u00df-SMMHC\u0394C95). Embryos with a single copy of CBF\u00df-SMMHCDC95 were viable and showed no defects in hematopoiesis, while embryos homozygous for the CBF\u00df-SMMHC\u0394C95 allele had hematopoietic defects and died in mid-gestation, similar to embryos with a single-copy of the full-length CBF\u00df-SMMHC\u0394C95. To identify gene expression changes induced by CBF\u00df-SMMHCDC95, we compared the gene expression profile in the blood cells of Cbfb+/+, Cbfb+/\u0394C95, and Cbfb\u0394C95/\u0394C95 embryonic day 12.5 (E12.5) mice. Cbfb+/\u0394C95 were mated together to generate Cbfb+/+, Cbfb+/\u0394C95, and Cbfb\u0394C95/\u0394C95 embryos. Blood from 8-10 E12.5 embryos of the same genotype was pooled, and RNA was isolated, labeled, and hybridized to Affymetrix Genechip mouse microarray (430 2.0) chips. 3 chips were used for each genotype.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42238", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42238/samples/"}