{"owner": "ArrayExpress Uploader", "pop_total": 0, "species": "mouse", "factors": [{"GSM1036016": {"CELL TYPE": "sorted  HSCs (lin-Mac-1 low  sca1+ckit+CD150+CD48-)", "VARIATION": "not specified", "DEVELOPMENTAL STAGE": "fetal"}}, {"GSM1036017": {"CELL TYPE": "sorted  HSCs (lin-Mac-1 low sca1+ckit+CD150+CD48-)", "VARIATION": "not specified", "DEVELOPMENTAL STAGE": "fetal"}}, {"GSM1036017": {"CELL TYPE": "sorted  HSCs (lin-Mac-1 low sca1+ckit+CD150+CD48-)", "VARIATION": "not specified", "DEVELOPMENTAL STAGE": "fetal"}}, {"GSM1036019": {"CELL TYPE": "sorted  HSCs (lin-sca1+ckit+CD150+CD48-)", "VARIATION": "wild type", "DEVELOPMENTAL STAGE": "adult"}}, {"GSM1036019": {"CELL TYPE": "sorted  HSCs (lin-sca1+ckit+CD150+CD48-)", "VARIATION": "wild type", "DEVELOPMENTAL STAGE": "adult"}}, {"GSM1036019": {"CELL TYPE": "sorted  HSCs (lin-sca1+ckit+CD150+CD48-)", "VARIATION": "wild type", "DEVELOPMENTAL STAGE": "adult"}}, {"GSM1036022": {"CELL TYPE": "sorted  HSCs (lin-sca1+ckit+CD150+CD48-)", "VARIATION": "Pu.1 knock-in (PU.1ki/ki)", "DEVELOPMENTAL STAGE": "adult"}}, {"GSM1036022": {"CELL TYPE": "sorted  HSCs (lin-sca1+ckit+CD150+CD48-)", "VARIATION": "Pu.1 knock-in (PU.1ki/ki)", "DEVELOPMENTAL STAGE": "adult"}}, {"GSM1036022": {"CELL TYPE": "sorted  HSCs (lin-sca1+ckit+CD150+CD48-)", "VARIATION": "Pu.1 knock-in (PU.1ki/ki)", "DEVELOPMENTAL STAGE": "adult"}}, {"GSM1036019": {"CELL TYPE": "sorted  HSCs (lin-sca1+ckit+CD150+CD48-)", "VARIATION": "wild type", "DEVELOPMENTAL STAGE": "adult"}}, {"GSM1036019": {"CELL TYPE": "sorted  HSCs (lin-sca1+ckit+CD150+CD48-)", "VARIATION": "wild type", "DEVELOPMENTAL STAGE": "adult"}}, {"GSM1036019": {"CELL TYPE": "sorted  HSCs (lin-sca1+ckit+CD150+CD48-)", "VARIATION": "wild type", "DEVELOPMENTAL STAGE": "adult"}}, {"GSM1036019": {"CELL TYPE": "sorted  HSCs (lin-sca1+ckit+CD150+CD48-)", "VARIATION": "wild type", "DEVELOPMENTAL STAGE": "adult"}}, {"GSM1036022": {"CELL TYPE": "sorted  HSCs (lin-sca1+ckit+CD150+CD48-)", "VARIATION": "Pu.1 knock-in (PU.1ki/ki)", "DEVELOPMENTAL STAGE": "adult"}}, {"GSM1036022": {"CELL TYPE": "sorted  HSCs (lin-sca1+ckit+CD150+CD48-)", "VARIATION": "Pu.1 knock-in (PU.1ki/ki)", "DEVELOPMENTAL STAGE": "adult"}}, {"GSM1036022": {"CELL TYPE": "sorted  HSCs (lin-sca1+ckit+CD150+CD48-)", "VARIATION": "Pu.1 knock-in (PU.1ki/ki)", "DEVELOPMENTAL STAGE": "adult"}}, {"GSM1036022": {"CELL TYPE": "sorted  HSCs (lin-sca1+ckit+CD150+CD48-)", "VARIATION": "Pu.1 knock-in (PU.1ki/ki)", "DEVELOPMENTAL STAGE": "adult"}}], "id": 7078, "ownerprofile_id": "arrayexpress_sid", "platform": 6, "summary_wrapped": "In blood, the transcription factor C/EBPa is essential for myeloid differentiation and has been implicated in regulating self-renewal of...", "geo_gse_id": "E-GEOD-42234", "owner_profile": "/profile/8773/arrayexpressuploader", "factor_count": 3, "sample_count": 17, "tags": ["cell", "liver"], "lastmodified": "Dec.12, 2014", "is_default": false, "geo_gds_id": "", "slug": "cebpa-controls-acquisition-and-maintenance-of-adul", "geo_id_plat": "E-GEOD-42234_A-AFFY-45", "name": "C/EBPa controls acquisition and maintenance of adult hematopoietic stem cell quiescence", "created": "Nov.12, 2014", "summary": "In blood, the transcription factor C/EBPa is essential for myeloid differentiation and has been implicated in regulating self-renewal of fetal liver hematopoietic stem cells (HSCs). However, its function in adult HSCs is unknown. Here, using an inducible knockout model, we found that C/EBPa deficient adult HSCs underwent a pronounced expansion with enhanced proliferation, characteristics resembling fetal liver HSCs. Consistently, transcription profiling of C/EBPa deficient HSCs revealed a gene expression program similar to fetal liver HSCs. Moreover we observed that age-specific C/EBPa expression correlated with its inhibitory effect on the HSC cell cycle. Mechanistically, we identified N-Myc as a C/EBPa downstream target. C/EBPa upregulation during HSC transition from an active fetal state to a quiescent adult state was accompanied by down-regulation of N-Myc, and loss of C/EBPa resulted in de-repression of NMyc. Our data establish that C/EBPa acts as a molecular switch between fetal and adult states of HSC in part via transcriptional repression of the proto-oncogene N-Myc. HSCs of Pu.1 knock-in (PU.1ki/ki) mice were used for RNA extraction and hybridization on Affymetrix microarrays. We compared these microarray samples with the corresponding wild type.", "source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42234", "sample_source": "http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42234/samples/"}