ArrayExpress Uploader07072peri TregLymphnodeperi naive TcellLymphnodeTreg precursor cellthymusTreg Foxp3+GFP+ CD24hithymusTreg Foxp3+GFP+ CD24intthymusTreg Foxp3+GFP+ CD24lowthymusTreg Foxp3-GFP- CD24hithymusTreg Foxp3-GFP- CD24intermediatethymusTreg Foxp3-GFP- CD24lowthymusperi TregLymphnodeperi naive TcellLymphnodeTreg precursor cellthymusTreg Foxp3+GFP+ CD24hithymusTreg Foxp3+GFP+ CD24intthymusTreg Foxp3+GFP+ CD24lowthymusTreg Foxp3-GFP- CD24hithymusTreg Foxp3-GFP- CD24intermediatethymusTreg Foxp3-GFP- CD24lowthymusperi TregLymphnodeperi naive TcellLymphnodeTreg precursor cellthymusTreg Foxp3+GFP+ CD24hithymusTreg Foxp3+GFP+ CD24intthymusTreg Foxp3+GFP+ CD24lowthymusTreg Foxp3-GFP- CD24hithymusTreg Foxp3-GFP- CD24intermediatethymusTreg Foxp3-GFP- CD24lowthymusarrayexpress_sid6We investigated at which stage of maturation commitment to a stable Foxp3-expressing phenotype takes place. We assessed stability of...23420886E-GEOD-42021/profile/8773/arrayexpressuploader227Dec.12, 2014FalseE-GEOD-42021_A-AFFY-45gene-expression-profiles-of-cd4sp-foxp3gfp-cd25-trGene expression profiles of CD4SP Foxp3GFP-CD25+ Treg precursors and Foxp3GFP-CD25- thymocytesNov.12, 2014We investigated at which stage of maturation commitment to a stable Foxp3-expressing phenotype takes place. We assessed stability of Foxp3 expression in thymic Foxp3+ Treg subsets of different maturity, defined by CD24 expression. Next we compared gene expression profiles of Foxp3+ Treg subsets (+) of different maturity (24lo, 24int, 24hi) and could identify a set of genes that were specifically up or downregulated in Foxp3+ Tregs, but not in Foxp3- conventional T cells, in a maturation-dependent manner. Gene expression of Foxp3+ Treg subsets (+) of different maturity (24lo, 24int, 24hi) compared to their Foxp3- conventional Tcell subsets (-) counterpart.http://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42021mousehttp://www.ebi.ac.uk/arrayexpress/experiments/E-GEOD-42021/samples/